Visan Diana-Carolina, Oprea Eliza, Radulescu Valeria, Voiculescu Ion, Biris Iovu-Adrian, Cotar Ani Ioana, Saviuc Crina, Chifiriuc Mariana Carmen, Marinas Ioana Cristina
Department of Organic Chemistry, Faculty of Pharmacy, University of Medicine and Pharmacy "Carol Davila", 6 Traian Vuia Street, 020956 Bucharest, Romania.
Department of Organic Chemistry, Biochemistry and Catalysis, Faculty of Chemistry, University of Bucharest, 4-12 Regina Elisabeta, 030018 Bucharest, Romania.
Pharmaceuticals (Basel). 2021 Nov 13;14(11):1159. doi: 10.3390/ph14111159.
This study aimed to establish the essential oil (EO) composition from young shoots of , , , and harvested from Romania and evaluate their antimicrobial and anti-virulence activity, as well as potential synergies with currently used antibiotics. The samples' EO average content varied between 0.62% and 1.02% (mL/100 g plant). The mono- and sesquiterpene hydrocarbons were dominant in the composition of the studied EOs. The antimicrobial activity revealed that the minimum inhibitory concentration (MIC) values for the tested EOs and some pure compounds known for their antimicrobial activity ranged from 6.25 to 100 µL/mL. The most intensive antimicrobial effect was obtained for the EO, which exhibited the best synergistic effect with some antibiotics against strains (i.e., oxacillin, tetracycline, erythromycin and gentamycin). The subinhibitory concentrations (sMIC) of the coniferous EOs inhibited the expression of soluble virulence factors (DN-ase, lipase, lecithinase, hemolysins, caseinase and siderophore-like), their efficiency being similar to that of the tested pure compounds, and inhibited the rhl gene expression in , suggesting their virulence-arresting drug potential.
本研究旨在确定从罗马尼亚采集的、、、和幼芽中提取的精油（EO）成分，评估其抗菌和抗毒力活性，以及与目前使用的抗生素的潜在协同作用。样品的EO平均含量在0.62%至1.02%（mL/100 g植物）之间。单萜和倍半萜碳氢化合物在所研究的EO成分中占主导地位。抗菌活性表明，测试的EO和一些已知具有抗菌活性的纯化合物的最低抑菌浓度（MIC）值在6.25至100 µL/mL之间。对EO的抗菌效果最为显著，它与一些抗生素对菌株（即苯唑西林、四环素、红霉素和庆大霉素）表现出最佳的协同作用。针叶树EO的亚抑菌浓度（sMIC）抑制了可溶性毒力因子（DN-酶、脂肪酶、卵磷脂酶、溶血素、酪蛋白酶和类铁载体）的表达，其效率与测试的纯化合物相似，并抑制了中的rhl基因表达，表明它们具有抑制毒力的药物潜力。
