Weekes Rebecca, Deppe Carola, Allnutt Theo, Boffey Caroline, Morgan Derek, Morgan Sarah, Bilton Mark, Daniels Roger, Henry Christine
Central Science Laboratory, YO41 1LZ Sand Hutton, York, UK.
Transgenic Res. 2005 Oct;14(5):749-59. doi: 10.1007/s11248-005-0943-2.
From 2000-2003 a range of Farm Scale Evaluation (FSE) trials were established in the UK to assess the effect of the release and management of herbicide tolerant (HT) crops on the abundance and diversity of farmland wildlife compared with their conventionally managed non-GM-equivalents. The objective of this research project was to investigate gene flow within the winter (WOSR) and spring oilseed rape (SOSR) FSE trials and to develop a statistical model for the prediction of cross-pollination frequency that can be used to evaluate current separation distance guidelines. Seed samples were collected from the non-GM half of the trial sites and were tested for evidence of cross-pollination from the GM HT halves using a quantitative PCR assay specific to the HT (bar) gene. Rates of cross-pollination were found to decrease rapidly with increasing distance from the GM source. The quantitative data were subjected to statistical analysis and a two-step model was found to provide the best fit for the data. Significant differences were found between the results for WOSR, SOSR and varietal association (VA) crops. The model predicted that the %GM content (including upper 95% confidence limits) of a sample taken at a distance of 50 m away from the GM source would be 0.04% (0.84%) for WOSR, 0.02% (0.39%) for SOSR, 0.77% (21.72%) for WOSR VA and 0.37% (5.18%) for SOSR VA. The data and models presented here are discussed in the context of necessary separation distances to meet various possible thresholds for adventitious presence of GM in OSR.
2000年至2003年期间，英国开展了一系列农场规模评估（FSE）试验，旨在评估与传统管理的非转基因等效作物相比，耐除草剂（HT）作物的种植与管理对农田野生动物数量和多样性的影响。本研究项目的目的是调查冬季（WOSR）和春季油菜（SOSR）FSE试验中的基因流动情况，并开发一种统计模型来预测异花授粉频率，该模型可用于评估当前的隔离距离指南。从试验场地的非转基因区域采集种子样本，使用针对HT（bar）基因的定量PCR检测法，检测样本中是否存在来自转基因HT区域的异花授粉证据。研究发现，异花授粉率随着与转基因源距离的增加而迅速下降。对定量数据进行了统计分析，发现两步模型最适合这些数据。WOSR、SOSR和品种关联（VA）作物的结果存在显著差异。该模型预测，在距离转基因源50米处采集的样本中，WOSR的转基因含量百分比（包括95%置信上限）为0.04%（0.84%），SOSR为0.02%（0.39%），WOSR VA为0.77%（21.72%），SOSR VA为0.37%（5.18%）。本文将结合满足油菜籽中GM偶然存在的各种可能阈值所需的隔离距离，对所呈现的数据和模型进行讨论。
