Peiris V, Heald J
Division of Hospital Infection, Central Public Health Laboratory, Colindale, London.
J Clin Pathol. 1992 Jun;45(6):532-4. doi: 10.1136/jcp.45.6.532.
The ability of Branhamella catarrhalis to cause nosocomial infections is a matter of some controversy. The API ZYM research kit for detecting 89 enzymes was used on 49 isolates of B catarrhalis to select enzymes of potential use in differentiating clinical isolates. Twenty nine enzymes were produced by all isolates (13 strongly positive) and many of these were esterases; 16 enzymes were not detected in any isolate (40 if a more stringent criterion was used). Twenty enzymes were selected to form a prototype biotyping panel which allowed 17 different patterns of reactivity to be recognised. Of the 49, 34 isolates were confined to the three commonest patterns. Only one isolate was untypable using this panel due to lack of reactivity. A kit with these 20 substrates may be sufficiently discriminatory to be useful in the rapid study of outbreaks of infection caused by B catarrhalis.
卡他莫拉菌引起医院感染的能力存在一定争议。使用用于检测89种酶的API ZYM研究试剂盒对49株卡他莫拉菌进行检测，以筛选出可能用于区分临床分离株的酶。所有分离株均可产生29种酶（13种强阳性），其中许多为酯酶；16种酶在任何分离株中均未检测到（若采用更严格标准则为40种）。选择20种酶组成一个原型生物分型板，可识别出17种不同的反应模式。在这49株分离株中，34株局限于三种最常见的模式。由于缺乏反应性，使用该分型板时仅有1株无法分型。含有这20种底物的试剂盒可能具有足够的鉴别力，有助于快速研究由卡他莫拉菌引起的感染暴发。
