改进的卡他莫拉菌脉冲场凝胶电泳方法
Improved pulsed field gel electrophoresis method for Moraxella catarrhalis.
作者信息
Pingault Nevada M, Lehmann Deborah, Riley Thomas V
机构信息
School of Biomedical, Biomolecular and Chemical Sciences, The University of Western Australia, Perth, Western Australia, Australia.
出版信息
J Microbiol Methods. 2008 Oct;75(2):344-5. doi: 10.1016/j.mimet.2008.05.021. Epub 2008 Jun 30.
PMID:18586342
Abstract
An improved PFGE method for the molecular typing of Moraxella catarrhalis is described. A modified PulseNet method using higher concentrations of EDTA and proteinase K, together with increased reagent volumes and incubation temperatures resulted in improved results and a more rapid turnaround time compared to PFGE methods currently used.
摘要
本文描述了一种改进的用于卡他莫拉菌分子分型的脉冲场凝胶电泳（PFGE）方法。与目前使用的PFGE方法相比，一种改良的PulseNet方法使用更高浓度的乙二胺四乙酸（EDTA）和蛋白酶K，同时增加试剂体积和孵育温度，结果得到了改善，周转时间更快。
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