Radford J R, Beighton D, House F
Hunterian Dental Research Unit, London Hospital Medical College, UK.
J Dent. 1992 Jun;20(3):145-51. doi: 10.1016/0300-5712(92)90126-w.
Bacterial plaque which had accumulated for 0.5, 1, 2, 4, 7, 14 and 28 days was cultured from the labial surface of one of the central incisor teeth in 15 monkeys (Macaca fascicularis). Each sampling area was defined by a metal well contained within a custom-made removable acrylic block. The number (log10) of the total colony forming units (c.f.u.) increased significantly from 3.40 to 6.02 between 0.5 and 28 days. Similarly the number of streptococci increased from 2.80 to 4.79 during the same period, although when expressed as a percentage of the total c.f.u., the proportions decreased from 34.9 per cent at 1 day to 6.9 per cent at 7 days and then remained at that level. The number of micro-aerophilic actinomyces increased throughout the period of plaque accumulation and comprised 15.3 per cent of the total c.f.u. at 28 days. Neisseriae were infrequently isolated from 0.5- and 1-day-old plaque and were not recovered at more than 1 per cent of the total c.f.u. during any period of plaque accumulation. The fastest doubling time (median) of the total c.f.u. was 1.9 h (range 1.3-18.5 h). The rapid development of dental plaque after tooth cleaning emphasizes the importance of regular and meticulous oral hygiene to control the accumulation of potentially periodontopathic and cariogenic bacteria.
从15只猕猴（食蟹猴）的一颗中切牙唇面培养累积0.5、1、2、4、7、14和28天的菌斑。每个采样区域由定制的可移除丙烯酸块内的金属孔界定。在0.5至28天期间，总菌落形成单位（c.f.u.）的数量（以10为底的对数）从3.40显著增加到6.02。同样，在此期间链球菌数量从2.80增加到4.79，尽管以总c.f.u.的百分比表示时，其比例从第1天的34.9%降至第7天的6.9%，然后保持在该水平。在菌斑累积期间，微需氧放线菌的数量持续增加，在28天时占总c.f.u.的15.3%。在0.5天和1天的菌斑中很少分离出奈瑟菌，在菌斑累积的任何时期，其在总c.f.u.中的回收率均不超过1%。总c.f.u.最快的倍增时间（中位数）为1.9小时（范围为1.3 - 18.5小时）。牙齿清洁后牙菌斑的快速形成强调了定期细致的口腔卫生对于控制潜在的牙周病和致龋菌积累的重要性。
