Ozkan Berna, Karabaş V Levent, Gündeş Sibel, Altintaş Ozgül, Etiler Nilay, Cağlar Yusuf
Department of Ophthalmology, Kocaeli University, School of Medicine, Kocaeli, Turkey.
J Cataract Refract Surg. 2005 Sep;31(9):1814-20. doi: 10.1016/j.jcrs.2005.06.033.
To compare the adherence of Staphylococcus epidermidis on AcrySof (Alcon) and poly(methyl methacrylate) (PMMA) intraocular lenses (IOLs) treated with vancomycin, teicoplanin, and cefuroxime.
Kocaeli University, School of Medicine, Kocaeli, Turkey.
The lenses were contaminated with S epidermidis solutions containing 10(8) colony-forming units. Intraocular lenses were placed in sterile triptic soy broth after being held in antibiotic solutions for 15 minutes. After that, sonication and vortex procedures were performed to remove all the attached bacteria that could not be removed by antibiotics. Ten microliters from each broth were taken and inoculated into sheep blood agar. The colonies were counted overnight. The statistical analyses were made using Mann-Whitney U and Kruskal-Wallis tests, and a P value less than 0.05 was considered statistically significant.
Regarding the inhibitory effect of antibiotics on bacterial adhesion, there was no statistically significant difference between AcrySof and PMMA lenses. In the cefuroxime group, the mean numbers of colony-forming units on AcrySof and PMMA lenses were 35.2 +/- 6.94 and 30.8 +/- 18.69, respectively (P>.05). In the teicoplanin group, the mean number of colony-forming units on AcrySof lenses was 100.1 +/- 20.97 and 70.6 +/- 43.75 on the PMMA lenses. The adherence in the vancomycin group was 245 +/- 273.74 colony-forming units for AcrySof lenses and 159.8 +/- 101.94 for PMMA lenses (P>.05). Regarding the inhibitory effect of antibiotics, cefuroxime was the most effective, followed by teicoplanin and vancomycin, respectively. Overall, the mean numbers of colony-forming units on the lenses that were held in cefuroxime, teicoplanin, and vancomycin solutions were 33.0 +/- 13.90, 85.35 +/- 36.66, and 202.4 +/- 205.74, respectively (P = .000).
The results suggest that cefuroxime, teicoplanin, and vancomycin significantly inhibit bacterial adherence to IOLs. The effect of cefuroxime on adherence inhibition was significantly higher than that of teicoplanin and vancomycin. Bacterial adherence is an important factor in bacterial virulence. Antibiotics, especially cefuroxime, can successfully inhibit bacterial adherence.
比较表皮葡萄球菌在经万古霉素、替考拉宁和头孢呋辛处理的丙烯酸酯（爱尔康）和聚甲基丙烯酸甲酯（PMMA）人工晶状体（IOL）上的黏附情况。
土耳其科贾埃利大学医学院。
将人工晶状体用含10⁸ 菌落形成单位的表皮葡萄球菌溶液污染。人工晶状体在抗生素溶液中放置15分钟后置于无菌胰蛋白胨大豆肉汤中。之后，进行超声处理和涡旋操作以去除抗生素无法去除的所有附着细菌。从每种肉汤中取10微升接种到绵羊血琼脂平板上。过夜计数菌落。采用Mann-Whitney U检验和Kruskal-Wallis检验进行统计学分析，P值小于0.05被认为具有统计学意义。
关于抗生素对细菌黏附的抑制作用，丙烯酸酯和PMMA人工晶状体之间无统计学显著差异。在头孢呋辛组，丙烯酸酯和PMMA人工晶状体上菌落形成单位的平均数分别为35.2±6.94和30.8±18.69（P>0.05）。在替考拉宁组，丙烯酸酯人工晶状体上菌落形成单位的平均数为100.1±20.97，PMMA人工晶状体上为70.6±43.75。在万古霉素组，丙烯酸酯人工晶状体的黏附数为245±273.74个菌落形成单位，PMMA人工晶状体为159.8±101.94个菌落形成单位（P>0.05）。关于抗生素的抑制作用，头孢呋辛最有效，其次分别是替考拉宁和万古霉素。总体而言，置于头孢呋辛、替考拉宁和万古霉素溶液中的人工晶状体上菌落形成单位的平均数分别为33.0±13.90、85.35±36.66和202.4±205.74（P = 0.000）。
结果表明头孢呋辛、替考拉宁和万古霉素可显著抑制细菌对人工晶状体的黏附。头孢呋辛对黏附抑制的效果显著高于替考拉宁和万古霉素。细菌黏附是细菌毒力的一个重要因素。抗生素，尤其是头孢呋辛，可成功抑制细菌黏附。
