Ophthalmology Department, Kocaeli University, School of Medicine, Kocaeli, Turkey.
Can J Ophthalmol. 2009 Dec;44(6):663-7. doi: 10.1139/i09-181.
To investigate and compare the effect of cefuroxime and moxifloxacin on adhesion of Staphylococcus epidermidis to intraocular lenses (IOLs).
Experimental study.
The 3-pieced hydrophobic acrylic lenses were contaminated with S. epidermidis (American Type Culture Collection 35983) solutions containing 108 colony-forming units. IOLs were inoculated into test tubes containing tryptic soy broth after being held in antibiotic solutions for 15 minutes. Sonication and vortex procedures were performed in order to remove all the remaining bacteria. From each tube 10 microL and 100 microL was taken and inoculated into sheep blood agar. The colonies were counted overnight. The statistical analyses were made using one-way ANOVA, Turkey Honestly Significant Differences test (HSD) and independent t tests, and a p value less than 0.05 was considered statistically significant.
Overall, the mean numbers of colony-forming units on the lenses that were held in control, cefuroxime, moxifloxacin 0.5 mg/0.1 mL and moxifloxacin 0.1 mg/0.1 mL solutions were 1398 (SE 10.01 x 10(3)), 29.9 (SE 1.16 x 10(3)), 0.23 (SD 0.04 x 10(3)), and 0.41 (SD 0.05 x 10(3)), respectively. The evaluation using one-way ANOVA and Turkey HSD tests revealed significant statistical differences among the groups (p = 0.000). The evaluation using independent t tests revealed significant statistical differences between the 2 moxifloxacin groups (p < 0.05).
Our results suggest that moxifloxacin and cefuroxime significantly inhibit bacterial adherence to IOLs. The effect of moxifloxacin on inhibition of bacterial adherence was significantly greater than that of cefuroxime. For this reason moxifloxacin might be considered as a better prophylactic agent.
研究并比较头孢呋辛和莫西沙星对表皮葡萄球菌黏附于人工晶状体（IOLs）的影响。
实验研究。
将 3 件式疏水性丙烯酸晶状体用含有 108 个菌落形成单位的表皮葡萄球菌（美国典型培养物保藏中心 35983）溶液污染。将 IOL 置于抗生素溶液中 15 分钟后，接种于含胰蛋白酶大豆肉汤的试管中。采用超声和涡旋程序以去除所有残留的细菌。从每个试管中取出 10 μL 和 100 μL 并接种到绵羊血琼脂上。过夜计数菌落。使用单因素方差分析、土耳其Honestly 显著差异检验（HSD）和独立 t 检验进行统计分析，p 值小于 0.05 被认为具有统计学意义。
总体而言，置于对照、头孢呋辛、莫西沙星 0.5 mg/0.1 mL 和莫西沙星 0.1 mg/0.1 mL 溶液中的晶状体上的菌落形成单位的平均数量分别为 1398（SE 10.01×10³）、29.9（SE 1.16×10³）、0.23（SD 0.04×10³）和 0.41（SD 0.05×10³）。使用单因素方差分析和土耳其 HSD 检验的评估显示组间存在显著的统计学差异（p=0.000）。使用独立 t 检验的评估显示，2 种莫西沙星组之间存在显著的统计学差异（p<0.05）。
我们的结果表明，莫西沙星和头孢呋辛显著抑制细菌黏附于 IOLs。莫西沙星抑制细菌黏附的效果明显大于头孢呋辛。因此，莫西沙星可能被视为更好的预防性药物。
