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七鳃鳗科(硬骨鱼纲:鲽形目)七种鱼类的染色体显带及18S核糖体DNA原位杂交分析

Chromosome banding and 18S rDNA in situ hybridization analysis of seven species of the family Achiridae (Teleostei: Pleuronectiformes).

作者信息

Carvalho de Azevedo Marisa Fagundes, Oliveira Claudio, Pardo Belén G, Martínez Paulino, Foresti Fausto

机构信息

Departamento de Morfologia, Laboratório de Biologia e Genética de Peixes. Instituto de Biociências, Universidade Estadual Paulista-UNESP, 18618-000 Botucatu, SP, Brazil.

出版信息

Genetica. 2005 Nov;125(2-3):125-32. doi: 10.1007/s10709-005-4921-7.

DOI:10.1007/s10709-005-4921-7
PMID:16247686
Abstract

Achiridae is an important family of the order Pleuronectiformes widely distributed in North, Central, and South America with freshwater and marine species. In the present study cytogenetic analyses comprising conventional and molecular techniques were carried out in seven species of this family. The following diploid numbers (2n) and fundamental numbers (FN) were obtained: Achirus declivis 2n=34, FN=52; Achirus lineatus 2n=40, FN=66; Catathyridium jenynsi 2n=40 and FN=50; Gymnachirus nudus 2n=36 and FN=50; Hypoclinemus mentalis 2n=38 and FN=54; Trinectes paulistanus 2n=42 and FN=52; and Trinectes sp. 2n=38 and FN=54. All species presented a single nucleolar organizer region (NOR) bearing chromosome pair and C-band positive segments mainly distributed at the pericentromeric position. The wide variation observed in chromosome number and FN suggests the occurrence of larger chromosome rearrangements in the family Achiridae if compared with other families of the same order.

摘要

无臂鳎科是鲽形目一个重要的科,广泛分布于北美洲、中美洲和南美洲,包括淡水和海洋物种。在本研究中,对该科的七个物种进行了包括传统和分子技术在内的细胞遗传学分析。获得了以下二倍体数(2n)和基数(FN):斜无臂鳎2n = 34,FN = 52;线纹无臂鳎2n = 40,FN = 66;珍氏无臂鲆2n = 40,FN = 50;裸无臂鳎2n = 36,FN = 50;颏下无臂鳎2n = 38,FN = 54;圣保罗无臂鲆2n = 42,FN = 52;以及无臂鲆属某物种2n = 38,FN = 54。所有物种均呈现出一对带有核仁组织区(NOR)的染色体,且C带阳性片段主要分布在着丝粒周围位置。与同一目的其他科相比,在无臂鳎科中观察到的染色体数和FN的广泛变异表明该科发生了较大的染色体重排。

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