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DNA自组装系统:以分支寡核苷酸为构建模块并通过芘激基缔合物带的形成进行监测

DNA self-assembling systems: branched oligonucleotides as building blocks and monitoring by pyrene excimer band formation.

作者信息

Lindegaard Dorthe, Babu B Ravindra, Wengel Jesper

机构信息

Nucleic Acid Center, Department of Chemistry, University of Southern Denmark, Odense M DK-5230, Denmark.

出版信息

Nucleosides Nucleotides Nucleic Acids. 2005;24(5-7):679-81. doi: 10.1081/ncn-200060247.

Abstract

The construction of a DNA self-assembling system created by four Y-shaped branched oligodeoxynucleotide building blocks has been studied. The assembly was verified by changes in the fluorescence emission spectra and revealed an additive effect in pyrene excimer band formation during DNA self-assembly.

摘要

对由四个Y形分支寡脱氧核苷酸构建块创建的DNA自组装系统的构建进行了研究。通过荧光发射光谱的变化验证了组装过程,并揭示了DNA自组装过程中芘激基缔合物带形成的加和效应。

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