Mohe Nikhil, Heinonen Petri, Sanghvi Yogesh S, Strömberg Roger
Division of Organic and Bioorganic Chemistry, MBB, Scheele Laboratory, Karolinska Institutet, Stockholm, Sweden.
Nucleosides Nucleotides Nucleic Acids. 2005;24(5-7):897-9. doi: 10.1081/ncn-200059257.
A fast and convenient procedure for synthesis of dinucleoside H-phosphonates is obtained through use of the novel polystyrene supported 5-carboxy-5-methyl-2-oxo-2-chloro-1,3,2-diaoxaphosphorinane reagent. Virtually quantitative H-phosphonate condensations are obtained leading to excellent isolatedyields and with only a simple filtration as the purification procedure. This provides for a convenient and high-yielding procedure that should be suited for solution-phase synthesis of oligonucleotides.
通过使用新型聚苯乙烯负载的5-羧基-5-甲基-2-氧代-2-氯-1,3,2-二氧磷杂环己烷试剂，获得了一种快速便捷的合成二核苷H-膦酸酯的方法。几乎定量的H-膦酸酯缩合反应能够实现，从而获得优异的分离产率，并且仅需简单过滤作为纯化步骤。这提供了一种方便且高产率的方法，适用于寡核苷酸的溶液相合成。
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