使用1-[(2-氯-4-甲基)苯基]-4-甲氧基哌啶-4-基（Ctmp）基团保护2'-羟基官能团并采用H-膦酸酯法进行寡核糖核苷酸的固相合成。
Solid phase synthesis of oligoribonucleotides using the 1-[(2-chloro-4-methyl)phenyl]-4-methoxypiperidin-4-yl (Ctmp) group for the protection of the 2'-hydroxy functions and the H-phosphonate approach.
作者信息
Sakatsume O, Ohtsuki M, Takaku H, Reese C B
机构信息
Department of Industrial Chemistry, Chiba Institute of Technology, Japan.
出版信息
Nucleic Acids Res. 1989 May 25;17(10):3689-97. doi: 10.1093/nar/17.10.3689.
Abstract
The solid phase synthesis of oligoribonucleotides using the H-phosphonate approach and the 1-[(2-chloro-4-methyl)phenyl]-4-methoxypiperidin-4-yl (Ctmp) and dimethoxytrityl (DMTr) groups, respectively, for the protection of the 2'- and 5'-hydroxy functions is described. The use of a new reagent, tris-(1,1,1,3,3,3-hexafluoro-2-propyl) phosphite for the preparation of nucleoside H-phosphonate units is also discussed in detail.
摘要
描述了使用H-膦酸酯方法以及分别使用1-[(2-氯-4-甲基)苯基]-4-甲氧基哌啶-4-基（Ctmp）和二甲氧基三苯甲基（DMTr）基团对2'-和5'-羟基官能团进行保护来固相合成寡核糖核苷酸。还详细讨论了使用一种新试剂亚磷酸三（1,1,1,3,3,3-六氟-2-丙基）酯来制备核苷H-膦酸酯单元。
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引用本文的文献
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2
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3
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Translational initiation in prokaryotes.原核生物中的翻译起始
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Solid phase synthesis of oligoribonucleotides using o-nitrobenzyl protection of 2'-hydroxyl via a phosphite triester approach.通过亚磷酸三酯法利用邻硝基苄基对2'-羟基进行保护的寡核糖核苷酸的固相合成。
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Solid phase synthesis of oligoribonucleotides using the o-nitrobenzyl group for 2'-hydroxyl protection and H-phosphonate chemistry.使用邻硝基苄基进行2'-羟基保护和H-膦酸酯化学法固相合成寡核糖核苷酸。
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