Sakatsume O, Ohtsuki M, Takaku H, Reese C B
Department of Industrial Chemistry, Chiba Institute of Technology, Japan.
Nucleic Acids Res. 1989 May 25;17(10):3689-97. doi: 10.1093/nar/17.10.3689.
The solid phase synthesis of oligoribonucleotides using the H-phosphonate approach and the 1-[(2-chloro-4-methyl)phenyl]-4-methoxypiperidin-4-yl (Ctmp) and dimethoxytrityl (DMTr) groups, respectively, for the protection of the 2'- and 5'-hydroxy functions is described. The use of a new reagent, tris-(1,1,1,3,3,3-hexafluoro-2-propyl) phosphite for the preparation of nucleoside H-phosphonate units is also discussed in detail.
描述了使用H-膦酸酯方法以及分别使用1-[(2-氯-4-甲基)苯基]-4-甲氧基哌啶-4-基(Ctmp)和二甲氧基三苯甲基(DMTr)基团对2'-和5'-羟基官能团进行保护来固相合成寡核糖核苷酸。还详细讨论了使用一种新试剂亚磷酸三(1,1,1,3,3,3-六氟-2-丙基)酯来制备核苷H-膦酸酯单元。