Kozlov Igor A, Dang Minh, Sikes Ken, Kotseroglou Theofilos, Barker David L, Zhao Chanfeng
Illumina, Inc., San Diego, California, USA.
Nucleosides Nucleotides Nucleic Acids. 2005;24(5-7):1037-41. doi: 10.1081/ncn-200059761.
A key factor influencing the quality of long oligonucleotides is the choice of controlled pore glass (CPG) which is used as a solid support during oligonucleotide synthesis. We studied the influence of CPG pore size on the quality of 75-mer oligonucleotides. Using electrophoresis and HPLC, we demonstrated failure modes that can occur at certain oligo lengths with 1000A pore size, and compared yield and purity of 75-mer oligos using 1000A and larger pore size CPG. We showed that oligonucleotides with much better quality are obtained using CPG with pore sizes of 1400A and larger. We also identified the key characteristics for CPG selection that lead to the best CPG performance.
影响长链寡核苷酸质量的一个关键因素是在寡核苷酸合成过程中用作固相载体的可控孔径玻璃（CPG）的选择。我们研究了CPG孔径对75聚体寡核苷酸质量的影响。通过电泳和高效液相色谱，我们证明了在某些寡核苷酸长度下使用1000埃孔径时可能出现的失败模式，并比较了使用1000埃及更大孔径CPG的75聚体寡核苷酸的产量和纯度。我们表明，使用孔径为1400埃及更大的CPG可获得质量更好的寡核苷酸。我们还确定了导致最佳CPG性能的CPG选择的关键特性。
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