Guo Ruoling, Zheng Qinguo
School of Life and Health Sciences, Aston University, Birmingham B4 7ET, UK.
Nucleosides Nucleotides Nucleic Acids. 2005;24(5-7):1081-3. doi: 10.1081/ncn-200059175.
HPLC MS/MS has shown great potential in the measurement of DNA oxidative damage. Its accuracy depends on the use of multiply isotopically labelled internal standards. In this report, multiply isotopically labelled (M + 4) guanine internal standards were prepared in the form of base, nucleoside, as well as DNA oligomer. To our knowledge, this is the first chemical synthesis of oligomers containing (M + 4) guanine, and we believe that they can be used to develop a procedure that can make further improvement to the existing analytical procedures.
高效液相色谱串联质谱法（HPLC MS/MS）在测量DNA氧化损伤方面已显示出巨大潜力。其准确性取决于多重同位素标记内标的使用。在本报告中，以碱基、核苷以及DNA寡聚体的形式制备了多重同位素标记（M + 4）鸟嘌呤内标。据我们所知，这是首次化学合成含（M + 4）鸟嘌呤的寡聚体，并且我们相信它们可用于开发一种能够对现有分析程序做出进一步改进的方法。
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