Vetkin D O, Vlaskin D N, Gaĭnulina E T, Ryzhikov S B, Taranchenko V F
Biofizika. 2005 Sep-Oct;50(5):793-6.
The peripheral binding site of horse serum cholinesterase (EC 3.1.1.7) for quaternary ligands was investigated by fluorescent probing with the use of ethidium bromide. Spectral evidence for the participation of the tryptophan indole group of the peripheral site of horse serum cholinesterase in the formation of a cholinesterase complex with ethidium bromide is presented. The mechanism of cholinesterase effect on ethidium bromide fluorescence is proposed.
利用溴化乙锭进行荧光探测，研究了马血清胆碱酯酶（EC 3.1.1.7）对季铵配体的外周结合位点。提供了光谱证据，证明马血清胆碱酯酶外周位点的色氨酸吲哚基团参与了与溴化乙锭形成胆碱酯酶复合物的过程。提出了胆碱酯酶对溴化乙锭荧光影响的机制。
