Zuĭkova O V, Osipova D A, Vikhliantsev I M, Malyshev S L, Udal'tsov S N, Podlubnaia Z A
Biofizika. 2005 Sep-Oct;50(5):797-802.
The isoform composition of myosin light chains and the extent of their phosphorylation in skeletal and cardiac muscles of ground squirrel Citellus undulatus in different periods of hibernation were studied. Regulatory myosin light chains of skeletal muscles of hibernating ground squirrels were completely dephosphorylated, while 25% of these light chains in active animals were phosphorylated. During hibernation, a shift of isoform composition of essential and regulatory skeletal muscle myosin light chains toward slower isoforms was observed, which is evidenced by the data obtained on m. psoas and on the totality of all skeletal muscles. In the atrial myocardium of hibernating ground squirrels, ventricular myosin light chains 1 (up to 60%) were registered. In contrast, during arousal of ground squirrels, in ventricular myocardium the appearance of atrial myosin light chains 1 (up to 30%) was revealed. A possible role of posttranslation changes in myosin light chains and their isoform shifts in the hibernation scenario is discussed.
研究了冬眠不同时期地松鼠（Citellus undulatus）骨骼肌和心肌中肌球蛋白轻链的同工型组成及其磷酸化程度。冬眠地松鼠骨骼肌的调节性肌球蛋白轻链完全去磷酸化，而活跃动物中这些轻链有25%被磷酸化。在冬眠期间，观察到必需和调节性骨骼肌肌球蛋白轻链的同工型组成向较慢同工型转变，这在腰大肌和所有骨骼肌总体的数据中得到了证实。在冬眠地松鼠的心房心肌中，检测到心室肌球蛋白轻链1（高达60%）。相反，在地松鼠苏醒期间，在心室心肌中发现了心房肌球蛋白轻链1（高达30%）的出现。讨论了肌球蛋白轻链翻译后变化及其同工型转变在冬眠过程中的可能作用。
