Maslov D V, Il'iash L V, Ostroumov E E, Pogosian S I, Fadeev V V
Biofizika. 2005 Sep-Oct;50(5):843-50.
The possibilities of diagnosis of the state of the phytoplankton photosynthetic apparatus by the method of nonlinear laser fluorimetry (saturation fluorimetry), which can be realized in remote sensing mode, were investigated. A procedure for the determination of the nonsaturated fluorescence parameter phi0, which is proportional to the concentration of chlorophyll a molecules, and the parameter A, which is a product of three photophysical parameters of chlorophyll a molecule in native chloroplast, was elaborated. Laboratory experiments with the axenic culture of eurihaline Thalassiosira weissflogii showed that the parameter A depends on the state of the photosynthetic apparatus of the alga, which was varied by either DCMU treatment or exposure to actinic light.
研究了通过非线性激光荧光法（饱和荧光法）诊断浮游植物光合装置状态的可能性，该方法可在遥感模式下实现。详细阐述了一种测定非饱和荧光参数phi0（与叶绿素a分子浓度成正比）以及参数A（天然叶绿体中叶绿素a分子的三个光物理参数的乘积）的程序。对广盐性威氏海链藻的无菌培养物进行的实验室实验表明，参数A取决于藻类光合装置的状态，这种状态可通过二氯苯基二甲基脲（DCMU）处理或照射光化光来改变。
