Klebanov G I, Chichuk T V, Osipov A N, Vladimirov Iu A
Biofizika. 2005 Sep-Oct;50(5):862-6.
The role of lipid peroxidation products formed in membranes of human blood leukocytes after irradiation with He-Ne laser was studied. It was found that low-intensity laser irradiation (0.3-1.6 J/cm2) leads to both cell activation and an increase in the content of lipid peroxidation products. The intensity of lipid peroxidation was analyzed by estimating the amount of TBA reactive products and lipid diene conjugates. Irradiation in the presence of an exogenous photosensitizer (protoporphyrin IX) enhanced the phenomena observed. The use of antioxidants (tocopherol and ionol) completely eliminated the laser-induced effects (changes in leukocyte activity and accumulation of lipid peroxidation products). These results can be explained by the fact that laser irradiation leads to the activation of lipid peroxidation in leukocyte membranes, which in turn enhances the response of cells to the stimulus (priming).
研究了氦氖激光照射后人血白细胞膜中形成的脂质过氧化产物的作用。发现低强度激光照射（0.3 - 1.6 J/cm²）会导致细胞活化以及脂质过氧化产物含量增加。通过估计TBA反应产物和脂质二烯共轭物的量来分析脂质过氧化的强度。在外源光敏剂（原卟啉IX）存在下进行照射增强了所观察到的现象。使用抗氧化剂（生育酚和离子醇）完全消除了激光诱导的效应（白细胞活性变化和脂质过氧化产物积累）。这些结果可以通过以下事实来解释，即激光照射导致白细胞膜中脂质过氧化的活化，这反过来又增强了细胞对刺激的反应（启动）。
