Matsunaga Y, Yamazaki S, Moritsugu Y, Kuwabara Y, Nishigaki M
National Institute of Health, Japan.
Kansenshogaku Zasshi. 1992 Apr;66(4):434-40. doi: 10.11150/kansenshogakuzasshi1970.66.434.
The IgM and IgG antibody capture methods of the enzyme-linked immunosorbent assay (ELISA) for human parvovirus B19 were performed using Horseradish peroxidase (HRPO)-labeled anti B19 monoclonal antibody. Serially obtained serum samples from one erythema infectiosum (E.I.) patient were examined at once by this methods. The dOD values of the IgM and IgG antibodies decreased on the typical curves according to the course of recovery. In the epidemic case of E.I. among students of one nurse school, 1) The first patients was estimated by comparing the change of dOD values of sera obtained at end of the epidemic and 1.5 months later. 2) In the pre-existing antibody positive persons, the dOD values of IgG antibody did not changed during the epidemic. 3) After the E.I. epidemic, and approximately 30% of the students were remained uninfected.
采用辣根过氧化物酶（HRPO）标记的抗B19单克隆抗体，进行人细小病毒B19酶联免疫吸附测定（ELISA）的IgM和IgG抗体捕获方法。用此方法对一名传染性红斑（E.I.）患者连续采集的血清样本进行一次性检测。IgM和IgG抗体的吸光度差值（dOD）值根据恢复过程在典型曲线上下降。在一所护士学校学生中发生的E.I.流行病例中，1）通过比较流行末期和1.5个月后采集的血清dOD值变化来估算首例患者。2）在预先存在抗体阳性的人群中，IgG抗体的dOD值在流行期间未发生变化。3）E.I.流行过后，约30%的学生仍未感染。
