Nada T, Ichiyama S, Iida E, Kadoya M, Takeuchi J, Ohta M
Department of Clinical Laboratory Medicine, Nagoya University Hospital.
Kansenshogaku Zasshi. 1992 Apr;66(4):465-9. doi: 10.11150/kansenshogakuzasshi1970.66.465.
Four strains of Escherichia coli O114:non-motile (NM) were isolated from patients supposed to have endemic diarrhea in 1989. The plasmid DNA profiles and restriction fragment patterns of chromosomal DNA digested with Not I analysed by pulsed-field gel electrophoresis (PFGE) of E. coli O114:NM strains were compared with those of other 9 strains of E. coli O114 isolated elsewhere (O114:H2, 1 strain; O114:H4, 2 strains; O114:H9, 2 strains; O114:H10, 1 strain; O114:H11, 1 strain; O114:H21, 1 strain; O114:H32, 1 strain). All of E. coli O114:NM strains showed the same plasmid DNA profile and chromosomal DNA fragment patterns. The strains of E. coli O114:NM and O114:H11, and O114:H21 and O114:H32 showed the same plasmid DNA profiles, respectively. On the other hand, these strains could be differentiated by the chromosomal DNA fingerprint patterns. The chromosomal DNA fragment pattern of all E. coli O114:NM strains is completely different from those of the other 9 control strains. We suggest that chromosomal DNA fingerprinting is useful for the epidemiological study of E. coli O114 associated endemic diarrhea.
1989年，从疑似患有地方性腹泻的患者中分离出4株大肠杆菌O114：无动力（NM）菌株。通过脉冲场凝胶电泳（PFGE）分析的大肠杆菌O114：NM菌株的质粒DNA图谱以及用Not I消化的染色体DNA的限制性片段模式，与其他9株从其他地方分离出的大肠杆菌O114（O114：H2，1株；O114：H4，2株；O114：H9，2株；O114：H10，1株；O114：H11，1株；O114：H21，1株；O114：H32，1株）的图谱进行了比较。所有大肠杆菌O114：NM菌株均显示出相同的质粒DNA图谱和染色体DNA片段模式。大肠杆菌O114：NM与O114：H11菌株，以及O114：H21与O114：H32菌株分别显示出相同的质粒DNA图谱。另一方面，这些菌株可以通过染色体DNA指纹图谱进行区分。所有大肠杆菌O114：NM菌株的染色体DNA片段模式与其他9株对照菌株的模式完全不同。我们认为染色体DNA指纹图谱对于大肠杆菌O114相关地方性腹泻的流行病学研究是有用的。
