Beutin L, Orskov I, Orskov F, Zimmermann S, Prada J, Gelderblom H, Stephan R, Whittam T S
Robert Koch-Institut des Bundesgesundheitsamtes, Berlin, Federal Republic of Germany.
J Infect Dis. 1990 Dec;162(6):1329-34. doi: 10.1093/infdis/162.6.1329.
Eighty-eight Escherichia coli strains of the enteropathogenic (EPEC) group O114 that were isolated from humans and animals in geographically different places and over more than 30 years were examined for virulence markers, O:H serotypes, and for electrophoretic types by multilocus enzyme electrophoresis. Four major genetically tightly related clusters of strains showed close correlation between electrophoretic types and other phenotypic characters. Cluster I contained 35 EPEC class II strains of serotypes O114:H9 and O114:H- and 5 enterotoxigenic E. coli belonging to O114:H21 and O114:H49. Clusters II and III comprised 36 O114:H4, O114:H32, and O114:H- strains; most were of doubtful pathogenicity except one Verotoxin-positive O114:H4 strain isolated from a human with diarrhea. Cluster IV contained 9 classic EPEC strains of serogroup O114:H2 that were characterized by localized adherence to HEp-2 cells and by the EPEC adherence factor.
对88株从不同地理位置、历经30多年从人和动物中分离出的肠致病性大肠杆菌（EPEC）O114群菌株进行了毒力标记、O:H血清型以及多位点酶电泳分析其电泳类型的检测。四个主要的遗传紧密相关菌株簇显示出电泳类型与其他表型特征之间存在密切关联。簇I包含35株血清型为O114:H9和O114:H-的EPEC II类菌株以及5株属于O114:H21和O114:H49的产肠毒素大肠杆菌。簇II和簇III包含36株O114:H4、O114:H32和O114:H-菌株；除了从一名腹泻患者分离出的一株产志贺毒素阳性的O114:H4菌株外，大多数菌株的致病性存疑。簇IV包含9株血清群为O114:H2的经典EPEC菌株，其特征为对HEp-2细胞的局部黏附以及具有EPEC黏附因子。
