Lu Xiang, Liu Zheng, Cui Yonghua
Department of Otorhinolaryngology, Tongji Hospital, Huazhong University of Science and Technology, Wuhan 430030, China.
Lin Chuang Er Bi Yan Hou Ke Za Zhi. 2005 Jul;19(14):633-5.
To compare the expression difference of transforming growth factor beta1 (TGF-beta1), matrix metalloproteinases1,7,9 (MMP-1,7,9 ) and tissue inhibitors of matrix metalloproteinases-1 (TIMP-1) protein among the chronic rhinosinusitis tissues, nasal polyps tissues and normal nasal mucosa tissues.
The protein expression of TGF-beta1, MMP-1, MMP-7, MMP-9 and TIMP-1 in chronic rhinosinusitis tissues from 22 patients, in nasal polyps tissues from 21 patients and in inferior turbinate tissues from 15 patients underwent nasal septum operation was detected by enzyme-linked immunosorbent assay (ELISA).
(1) In chronic rhinosinusitis and nasal polyps tissues, compared with controls, the expression of TGF-beta1, MMP-7, MMP-9, TIMP-1 protein was significant increased (P < 0.05); (2) The expression of TGF-beta1 and TIMP-1 in chronic rhinosinusitis tissues was significant increased versus that in nasal polyps tissues (P < 0.05); (3) Concentration of MMP-7 protein in nasal polyps tissues was found significant higher than that in chronic rhinosinusitis tissues (P < 0.01).
Different TGF-beta1, MMP-7, TIMP-1 protein level in chronic rhinosinusitis and nasal polyps tissues might be the molecular basis of the different histopathological character of chronic rhinosinusitis and nasal polyp tissues.
比较转化生长因子β1（TGF-β1）、基质金属蛋白酶1、7、9（MMP-1、7、9）和基质金属蛋白酶组织抑制剂-1（TIMP-1）蛋白在慢性鼻窦炎组织、鼻息肉组织和正常鼻黏膜组织中的表达差异。
采用酶联免疫吸附测定法（ELISA）检测22例慢性鼻窦炎患者的慢性鼻窦炎组织、21例鼻息肉患者的鼻息肉组织以及15例行鼻中隔手术患者的下鼻甲组织中TGF-β1、MMP-1、MMP-7、MMP-9和TIMP-1的蛋白表达。
（1）在慢性鼻窦炎和鼻息肉组织中，与对照组相比，TGF-β1、MMP-7、MMP-9、TIMP-1蛋白表达显著增加（P < 0.05）；（2）慢性鼻窦炎组织中TGF-β1和TIMP-1的表达显著高于鼻息肉组织（P < 0.05）；（3）发现鼻息肉组织中MMP-7蛋白浓度显著高于慢性鼻窦炎组织（P < 0.01）。
慢性鼻窦炎和鼻息肉组织中TGF-β1、MMP-7、TIMP-1蛋白水平不同可能是慢性鼻窦炎和鼻息肉组织不同组织病理学特征的分子基础。
