Li Ya-juan, Zhuang Hui, Li Jie, Dong Qing-ming, Chen Ya-jie, Niu Jun-qi, Ma Wei-min, Zhao Wei, Zhao Bao-an, Zhong Jin-qun
Department of Microbiology, Peking University Health Science Center, Beijing 100083, China.
Zhonghua Gan Zang Bing Za Zhi. 2005 Oct;13(10):724-9.
To study hepatitis B virus (HBV) genotype and subtype distribution and its clinical significance in HBV-infected patients.
We used type/subtype-specific primers and PCR to detect HBV genotype and subtype of 445 HBV-infected patients from Beijing, Changchun, Hanchuan Shenzhen, Qingyuan and Nanjing, including 7 acute hepatitis (AH), 36 asymptomatic HBV carriers (ASC), 352 chronic hepatitis (CH), 28 liver cirrhosis (LC), and 22 hepatocellular carcinoma (HCC) cases. Genotyping results were confirmed by PCR product sequencing.
Among 445 HBV-infected patients, the proportions of genotype B, C, and B/C were 32.6% (145/445), 53.7% (239/445), and 13.7% (61/445), respectively. In genotype C, 13 (5.4%) were subtype C1, 135 (56.5%) were subtype C2, and the remaining 91 (38.1%) were neither C1 nor C2. In genotype B, 100 (69.0%) were subtype Ba, 25 (17.2%) subtype Bj, and the other 20 (13.8%) were neither Ba nor Bj. In genotype B/C, 15 (24.6%) were Ba/C2, 8 (13.1%) Bj/C2, 6 (9.8%) Ba/C1, 3 (4.9%) Bj/C1, 11 (18.0%) Ba/neither C1 nor C2, 7 (11.5%) Bj/neither C1 nor C2, and 6 (9.8%) neither Ba nor Bj/neither C1 nor C2, 2 (3.3%) neither Ba nor Bj/C1, 3 (4.9%) neither Ba nor Bj/C2. The HBV genotype and subtype distribution we found exhibited significant differences in the various clinical types of HBV infection tested, and showed that genotype C was predominant among patients with liver cirrhosis (78.6%) and hepatocellular carcinoma (86.4%) while genotype B was predominant in asymptomatic carriers (72.2%). In addition, genotype and subtype distribution showed no significant differences between male and female patients, but genotype and subtype distribution showed significant differences in patients positive or negative with HBeAg.
Subtypes Ba and C2 are predominant in patients with hepatitis B from these 6 cities, and genotype C may be associated with the development of liver cirrhosis and hepatocellular carcinoma.
研究乙型肝炎病毒（HBV）基因型和亚型分布及其在HBV感染患者中的临床意义。
我们使用型/亚型特异性引物和聚合酶链反应（PCR）检测了来自北京、长春、汉川、深圳、清远和南京的445例HBV感染患者的HBV基因型和亚型，其中包括7例急性肝炎（AH）、36例无症状HBV携带者（ASC）、352例慢性肝炎（CH）、28例肝硬化（LC）和22例肝细胞癌（HCC）患者。通过PCR产物测序确认基因分型结果。
在445例HBV感染患者中，基因型B、C和B/C的比例分别为32.6%（145/445）、53.7%（239/445）和13.7%（61/445）。在基因型C中，13例（5.4%）为C1亚型，135例（56.5%）为C2亚型，其余91例（38.1%）既不是C1也不是C2亚型。在基因型B中，100例（69.0%）为Ba亚型，25例（17.2%）为Bj亚型，另外20例（13.8%）既不是Ba也不是Bj亚型。在基因型B/C中，15例（24.6%）为Ba/C2，8例（13.1%）为Bj/C2，6例（9.8%）为Ba/C1，3例（4.9%）为Bj/C1，11例（18.0%）为Ba/既不是C1也不是C2，7例（11.5%）为Bj/既不是C1也不是C2，6例（9.8%）既不是Ba也不是Bj/既不是C1也不是C2，2例（3.3%）既不是Ba也不是Bj/C1，3例（4.9%）既不是Ba也不是Bj/C2。我们发现的HBV基因型和亚型分布在检测的各种HBV感染临床类型中存在显著差异，并且表明基因型C在肝硬化患者（78.6%）和肝细胞癌患者（86.4%）中占主导地位，而基因型B在无症状携带者中占主导地位（72.2%）。此外，基因型和亚型分布在男性和女性患者之间没有显著差异，但在HBeAg阳性或阴性患者中基因型和亚型分布存在显著差异。
Ba和C2亚型在这6个城市的乙型肝炎患者中占主导地位，并且基因型C可能与肝硬化和肝细胞癌的发生有关。
