[中国12个城市慢性乙型肝炎病毒感染患者中乙型肝炎病毒基因型的分布]

[Distribution of hepatitis B virus genotypes in patients with chronic hepatitis B virus infection among 12 cities in China].

作者信息

Gao Jun-wei, Li Ya-juan, Zhuang Hui, Li Jie, Wang Jia, Dong Qing-ming, Chen Ya-jie, Niu Jun-qi, Ma Wei-Min, Zhao Wei, Zhao Bao-an, Zhong Jin-qun

机构信息

Department of Microbiology, Peking University Health Science Center, Beijing 100083, China.

出版信息

Zhonghua Liu Xing Bing Xue Za Zhi. 2007 Apr;28(4):315-8.

PMID:17850692

Abstract

OBJECTIVE

To investigate the distribution of hepatitis B virus (HBV) genotypes in patients with chronic HBV infection among 11 cities of China.

METHODS

A total of 1214 serum samples from patients with chronic HBV infection were collected in 11 cities of China, including Beijing, Qingyuan, Shenzhen, Shijiazhuang, Hanchuan, Nanjing, Changchun, Liaocheng, Jinan, Ningbo and Wenzhou. Genotypes of the 1214 HBV strains were identified by PCR method with type specific primers. Parts of the results were confirmed by direct sequencing analysis of PCR products.

RESULTS

Among the 1214 patients with chronic HBV infection, 0.7% (9/1214)were genotype A, 28.4% (345/1214)genotype B, 58.4% (709/1214) genotype C, and 12.4% (151/1214) genotype B and genotype C mixed infection. No other genotypes were found. Genotype C was predominant in the northern part of China, such as Changchun, Beijing, Shijiazhuang,while genotype B was more commonly seen in south of China. 71.4% (20/28) for patients from Qingyuan and 63.6% (70/110) from Shenzhen were infected with genotype B.

CONCLUSION

HBV genotypes had distinct geographic distribution. Genotype B and C the predominant strains in patients with chronic HBV infection in China. Genotype C was predominantly identified in the northern part of China versus genotype B the south.

摘要

目的

调查中国11个城市慢性乙型肝炎病毒（HBV）感染者中HBV基因型的分布情况。

方法

在中国11个城市（包括北京、清远、深圳、石家庄、汉川、南京、长春、聊城、济南、宁波和温州）收集了1214例慢性HBV感染者的血清样本。采用型特异性引物的PCR方法鉴定1214株HBV的基因型。部分结果通过对PCR产物的直接测序分析进行确认。

结果

在1214例慢性HBV感染者中，0.7%（9/1214）为A型，28.4%（345/1214）为B型，58.4%（709/1214）为C型，12.4%（151/1214）为B型和C型混合感染。未发现其他基因型。C型在中国北方地区占主导地位，如长春、北京、石家庄，而B型在中国南方更为常见。清远的患者中71.4%（20/28）感染B型，深圳的患者中63.6%（70/110）感染B型。