Cannata S M, Bernardini S, Filoni S
Dipartimento di Biologia, II Università di Roma, Italy.
J Exp Zool. 1992 Jul 1;262(4):446-53. doi: 10.1002/jez.1402620412.
The regenerative capacity of larval Xenopus laevis hindlimbs amputated through the tarsalia at different stages of development and explanted in vitro was tested. In the first experimental series hindlimb stumps from stage 53, 54, 55, and 57 larvae (according to Nieuwkoop and Faber, '56) were cultured in Leibovitz's L-15 medium supplemented with 10% FCS, and 0.04 U of insulin and 10(-8) mg of L-thyroxine per ml of medium. Results showed that the distal part of the limb stumps from stages 53, 54, and 55 formed a regeneration blastema composed of proliferating mesenchymal cells beneath a typical apical cap. No blastema was formed in the proximal part of the stump. In limb stumps from stage 57, a regeneration blastema did not form either in the proximal or in the distal part of the stump. In a second experimental series, hindlimb stumps from stage 55 larvae, denervated 5 days prior to amputation in order to eliminate any residual neurotrophic factor, were cultured in a simplified L-15 medium containing 2% FCS and lacking insulin and thyroxine. Results showed that also in these experimental conditions the stumps from stage 55 formed a conical regeneration blastema at the distal tip. The blastema cells duplicated their own DNA and divided. At the proximal extremity no regeneration blastema was formed. In the same culture medium, the stumps of larvae at stage 57 did not form a regeneration blastema.(ABSTRACT TRUNCATED AT 250 WORDS)
对处于不同发育阶段、通过跗骨切断的非洲爪蟾幼体后肢进行体外移植，测试其再生能力。在第一个实验系列中，将53、54、55和57期幼体（根据Nieuwkoop和Faber，'56）的后肢残端在补充有10%胎牛血清、每毫升培养基含0.04单位胰岛素和10⁻⁸毫克L-甲状腺素的Leibovitz's L-15培养基中培养。结果显示，53、54和55期肢体残端的远端部分形成了由典型顶端帽下方增殖的间充质细胞组成的再生芽基。残端近端未形成芽基。在57期肢体残端中，残端近端和远端均未形成再生芽基。在第二个实验系列中，将55期幼体的后肢残端在截肢前5天去神经支配以消除任何残留的神经营养因子，然后在含有2%胎牛血清且不含胰岛素和甲状腺素的简化L-15培养基中培养。结果显示，在这些实验条件下，55期的残端在远端尖端也形成了圆锥形再生芽基。芽基细胞复制自身DNA并分裂。在近端未形成再生芽基。在相同培养基中，57期幼体的残端未形成再生芽基。（摘要截短于250字）
