Zeytunlu Murat, Korkut Mustafa, Akgün Erhan, Firat Ozgür, Aynaci Murat, Içöz Gökhan, Kiliç Murat, Ersin Sinan, Ozütemiz Omer
Department of Surgery, Ege University, Izmir, Turkey.
Turk J Gastroenterol. 2004 Dec;15(4):243-9.
BACKGROUND/AIMS: In this study two calcium channel blockers (CCB), diltiazem and verapamil, which demonstrate their effects on two different receptor blockage mechanisms, were assessed comparatively in an experimental colitis model regarding the local and systemic effect spectrum.
Eighty male Swiss albino rats were divided into eight groups (n:10 each): Group I) colitis was induced with 1 ml 4% acetic acid without any medication. Group II) Sham group. Group III) Intra-muscular (IM) diltiazem was administered daily for five days before inducing colitis. Group IV) IM verapamil was administered daily for five days before inducing colitis. Group V) Transrectal (TR) diltiazem was administered with enema daily for two days before inducing colitis. Group VI) TR saline was administered four hours before inducing colitis. Group VII) TR diltiazem was administered with enema four hours before inducing colitis. Group VIII) TR verapamil was administered with enema four hours before inducing colitis. All subjects were sacrificed 48 hours after the colitis induction. The distal colon segment was assessed macroscopically and microscopically for the grade of damage, and myeloperoxidase (MPO) activity was measured.
All the data of the control colitis group (group I), including the microscopic, macroscopic and MPO activity measurements, were significantly higher than in the groups in which verapamil and diltiazem were administered over seven days (3.100+/-0.7379 to 1.300+/-0.9487 and 1.600+/-0.9661) (p<0.05). The data of the Sham group, group II, were less than the other groups in which colitis was induced (p<0.05). For the local effect spectrum, after the assessment of groups V-VIII, the control colitis group (group I) and group VI had significantly higher values than the others (3.300+/-0.4830 to 1.800+/-0.6325 and 1.700+/-0.8233 (p<0.05).
Calcium channel blockage has systemic and local effects on the colitis model.
背景/目的：在本研究中，比较评估了两种通过不同受体阻断机制发挥作用的钙通道阻滞剂（CCB），即地尔硫䓬和维拉帕米，在实验性结肠炎模型中的局部和全身效应谱。
80只雄性瑞士白化大鼠分为八组（每组n = 10）：第一组）用1 ml 4%乙酸诱导结肠炎，不给予任何药物。第二组）假手术组。第三组）在诱导结肠炎前连续五天每日肌肉注射地尔硫䓬。第四组）在诱导结肠炎前连续五天每日肌肉注射维拉帕米。第五组）在诱导结肠炎前连续两天每日经直肠灌肠给予地尔硫䓬。第六组）在诱导结肠炎前4小时经直肠给予生理盐水。第七组）在诱导结肠炎前4小时经直肠灌肠给予地尔硫䓬。第八组）在诱导结肠炎前4小时经直肠灌肠给予维拉帕米。所有实验对象在诱导结肠炎后48小时处死。对远端结肠段进行宏观和微观损伤程度评估，并测量髓过氧化物酶（MPO）活性。
对照结肠炎组（第一组）的所有数据，包括微观、宏观和MPO活性测量值，均显著高于维拉帕米和地尔硫䓬给药七天的组（3.100±0.7379至1.300±0.9487和1.600±0.9661）（p<0.05）。假手术组（第二组）的数据低于其他诱导结肠炎的组（p<0.05）。对于局部效应谱，在评估第五组至第八组后，对照结肠炎组（第一组）和第六组的值显著高于其他组（3.300±0.4830至1.800±0.6325和1.700±0.8233（p<0.05）。
钙通道阻断对结肠炎模型有全身和局部作用。
