Department of Pharmacology, Assam Medical College, Dibrugarh, India.
Indian J Pharmacol. 2011 Feb;43(1):18-21. doi: 10.4103/0253-7613.75660.
Ulcerative colitis and Crohn's disease are chronic recurrent inflammatory bowel disease (IBD) of unknown origin. Oxidative stress is believed to be a key factor in the pathogenesis and perpetuation of the mucosal damage in IBD.
Ethanolic extract of Fragaria vesca (EFFV) fruits was prepared by percolation method and subjected to oral toxicity testing using OECD guidelines. Albino rats were pretreated orally for 5 days with 3% gum acacia in control, EFFV 500 mg/kg in test and 5-aminosalisylic acid (5-ASA) 100 mg/kg in standard groups. Colitis was induced by transrectal administration of 4% acetic acid on 5(th) day. All the animals were sacrificed with ether overdose 48 hours after colitis induction, and 10 cm colon segment was resected from proximal end. Colon was weighed (for disease activity index) and scored macroscopically and microscopically after histological staining. Biochemical assessments included myeloperoxidase (MPO) and tissue catalase (CAT), glutathione (GSH) and superoxide dismutase (SOD) measurements. Statistical analysis was done using one-way analysis of variance (ANOVA) followed by Dunnett's "t" test.
EFFV showed significant (P < 0.05) prevention of increase in colon weight and disease activity index along with decrease in macroscopic and microscopic lesion score as compared to control group. Significant improvement was observed in the levels of MPO, CAT and SOD, except GSH (P < 0.05). However, the effect of EFFV was significantly less than 5-ASA (P < 0.05).
EFFV at 500 mg/kg showed significant amelioration of experimentally induced IBD, which may be attributed to its antioxidant and anti-inflammatory properties.
溃疡性结肠炎和克罗恩病是病因不明的慢性复发性炎症性肠病（IBD）。氧化应激被认为是 IBD 黏膜损伤发病机制和持续存在的关键因素。
采用渗漉法制备悬钩子属植物乙醇提取物（EFFV），并按照 OECD 指南进行口服毒性试验。在对照组中，用 3%阿拉伯胶对白化大鼠进行 5 天的预处理，在试验组中用 500mg/kg 的 EFFV 预处理，在标准组中用 100mg/kg 的 5-氨基水杨酸预处理。在第 5 天通过直肠给予 4%乙酸诱导结肠炎。在结肠炎诱导后 48 小时，所有动物均用乙醚过量处死，并从近端切除 10cm 结肠段。对结肠进行称重（用于疾病活动指数），并在组织染色后进行宏观和微观评分。生化评估包括髓过氧化物酶（MPO）和组织过氧化氢酶（CAT）、谷胱甘肽（GSH）和超氧化物歧化酶（SOD）的测量。采用单因素方差分析（ANOVA）进行统计分析，然后采用 Dunnett 的“t”检验。
与对照组相比，EFFV 显著（P<0.05）预防了结肠重量和疾病活动指数的增加，同时减少了宏观和微观病变评分。与对照组相比，MPO、CAT 和 SOD 的水平显著改善（P<0.05），但 GSH 除外（P<0.05）。然而，EFFV 的效果明显低于 5-ASA（P<0.05）。
EFFV 500mg/kg 显著改善了实验诱导的 IBD，这可能归因于其抗氧化和抗炎特性。
