May Chr Albrecht, Lütjen-Drecoll Elke, Narfström Kristina
Department of Anatomy, Friedrich-Alexander University Erlangen-Nürnberg, Erlangen, Germany.
Curr Eye Res. 2005 Oct;30(10):855-62. doi: 10.1080/02713680591006219.
The purpose of this study was to investigate morphological changes of the anterior segment of the eye in Abyssinian cats with progressive rod-cone degeneration and to correlate them with blood flow data obtained in the same animals.
Sections of the left eyes of six normal cats and of eight cats with different stages of hereditary retinal degeneration were prepared for transmission and scanning electron microscopy. Tangential and sagittal sections were also stained with antibodies against substance P, tyrosine hydroxylase, panneuronal marker PGP9.5, nitric oxide synthase, synaptophysin, and smooth muscle alpha-actin.
In Abyssinian cats with hereditary rod-cone degeneration, significant changes were observed in the iris consisting of irregularities in the vascular wall of smaller arteries without changes in their innervation pattern. The ciliary processes were shorter than in normal cats, and their structure appeared more compact and retracted. Slight changes were also observed in the anterior part of the ciliary epithelium. The anterior chamber angle region did not appear to be affected.
Clear morphological correlations to the physiological blood flow data were observed in the anterior eye segment, pointing not only to functional but also morphological vascular abnormalities in this animal model for retinitis pigmentosa.
本研究旨在调查患有进行性视杆 - 视锥细胞变性的阿比西尼亚猫眼前节的形态变化，并将其与在同一动物身上获得的血流数据相关联。
制备了六只正常猫和八只患有不同阶段遗传性视网膜变性的猫的左眼切片，用于透射电子显微镜和扫描电子显微镜检查。还使用针对P物质、酪氨酸羟化酶、泛神经元标志物PGP9.5、一氧化氮合酶、突触素和平滑肌α - 肌动蛋白的抗体对切线和矢状切片进行染色。
在患有遗传性视杆 - 视锥细胞变性的阿比西尼亚猫中，虹膜出现了显著变化，表现为小动脉血管壁不规则，但其神经支配模式未改变。睫状体比正常猫的短，其结构显得更紧凑且回缩。在睫状上皮前部也观察到轻微变化。前房角区域似乎未受影响。
在眼前节观察到与生理血流数据明显的形态学相关性，这不仅表明在这种视网膜色素变性动物模型中存在功能性血管异常，还存在形态学血管异常。
