衰老过程中大鼠器官DNA中8-羟基脱氧鸟苷水平的变化。

Changes of 8-hydroxydeoxyguanosine levels in rat organ DNA during the aging process.

作者信息

Sai K, Takagi A, Umemura T, Hasegawa R, Kurokawa Y

机构信息

Division of Toxicology, National Institute of Hygienic Sciences, Tokyo, Japan.

出版信息

J Environ Pathol Toxicol Oncol. 1992 May-Jun;11(3):139-43.

PMID:1625183

Abstract

We investigated the accumulation of oxidative DNA damage during the aging process by using 8-hydroxydeoxyguanosine (8-OH-dG) as a marker. The 8-OH-dG is one of the oxidative DNA damage products and is supposed to be a critical factor in carcinogenesis involving oxygen radicals. The 8-OH-dG levels in the DNA of liver, kidney, brain, lung, and spleen were measured in male and female F344 rats 6- to 30-month-old. The 8-OH-dG levels in the liver and kidney DNA of male rats increased significantly with age, but did not change in brain, lung, and spleen. Similarly, the 8-OH-dG levels in the liver and kidney DNA of female rats significantly increased with age, while changes in the brain, lung, and spleen DNA were much smaller. These results indicate that the accumulation of oxidative DNA damage during the aging process varies among organs, with slight sex difference.

摘要

我们以8-羟基脱氧鸟苷（8-OH-dG）作为标志物，研究了衰老过程中氧化性DNA损伤的积累情况。8-OH-dG是氧化性DNA损伤产物之一，被认为是涉及氧自由基的致癌作用中的关键因素。我们测定了6至30月龄雄性和雌性F344大鼠肝脏、肾脏、大脑、肺和脾脏DNA中的8-OH-dG水平。雄性大鼠肝脏和肾脏DNA中的8-OH-dG水平随年龄显著增加，但在大脑、肺和脾脏中未发生变化。同样，雌性大鼠肝脏和肾脏DNA中的8-OH-dG水平也随年龄显著增加，而大脑、肺和脾脏DNA的变化则小得多。这些结果表明，衰老过程中氧化性DNA损伤的积累在不同器官间存在差异，且存在轻微的性别差异。