Kirby J D, Classen H L, Smyth J R, Froman D P
Department of Animal Sciences, Oregon State University, Corvallis 97331.
J Reprod Fertil. 1992 May;95(1):79-86. doi: 10.1530/jrf.0.0950079.
Male fowl were immunized intravenously (i.v.) or intramuscularly (i.m.) with spermatozoa to assess the effects of immunity to spermatozoa on fertility. Histological and immunofluorescence evaluations of testis and ductus deferens tissues after 24 weeks of immunizations revealed immune cell infiltration and immunoglobulin associated with spermatozoa. The long-term immunization regimen resulted in significant antisperm antibody titres in the immunized groups. When semen from i.v.-immunized males was used to inseminate females, fertility over 7 days was reduced (P less than 0.05). A subsequent experiment using a 10-week i.v. immunization scheme also led to high antisperm titres. Spermatozoa from these males were characterized by lower fertility and duration of fertility than those of controls (P less than 0.05). As in mammals, a reduction in fertility may result from exposure of avian males to sperm antigens.
为评估精子免疫对生育能力的影响，对雄性家禽进行静脉内（i.v.）或肌肉内（i.m.）精子免疫。免疫24周后，对睾丸和输精管组织进行组织学和免疫荧光评估，结果显示存在免疫细胞浸润以及与精子相关的免疫球蛋白。长期免疫方案导致免疫组出现显著的抗精子抗体滴度。当使用静脉免疫雄性的精液对雌性进行授精时，7天内的生育能力降低（P<0.05）。随后一项采用10周静脉免疫方案的实验也导致了高抗精子滴度。与对照组相比，这些雄性的精子生育能力较低且生育持续时间较短（P<0.05）。与哺乳动物一样，雄性禽类接触精子抗原可能导致生育能力下降。
