刺菠萝碱对伤口愈合的促进作用及其对成纤维细胞的影响
[Enhancement of wound healing by taspine and its effect on fibroblast].
作者信息
Dong Yalin, He Langchong, Chen Fang
机构信息
School of medicine, Xi' an Jiaotong University, Xi' an 71006.
出版信息
Zhong Yao Cai. 2005 Jul;28(7):579-82.
OBJECTIVE
To study the effect of taspine on enhancement of skin wound healing and its effect on fibroblast proliferation and autocrine.
METHODS
The plerosis effect of taspine on experimental skin wound was observed in vivo. Different concentrations of taspine were added in vitro and MTT technique was applied to observe its effect on fibroblast proliferation, the levels of transforming growth factor-beta1 (TGF-13P) and epidermal growth factor (EGF) were determined by ELISA.
RESULTS
In vivo, exo-applied taspine 300 microg and 150 microg accelerated the recovery of skin wound. In vitro, 0.50-0.4 microg/ml taspine could increase autocrine of TGF-beta1and EGF by fibroblast, but it showed no effect on L929 fibroblast proliferation.
CONCLUSIONS
Taspine enhances wound healing by increasing the autocrine of TGF-beta1 and EGF by fibroblast.
目的
研究紫堇定碱对促进皮肤伤口愈合的作用及其对成纤维细胞增殖和自分泌的影响。
方法
在体内观察紫堇定碱对实验性皮肤伤口的愈合作用。体外加入不同浓度的紫堇定碱，采用MTT法观察其对成纤维细胞增殖的影响，用ELISA法测定转化生长因子-β1（TGF-β1）和表皮生长因子（EGF）的水平。
结果
在体内，外用300μg和150μg紫堇定碱可加速皮肤伤口的愈合。在体外，0.50 - 0.4μg/ml紫堇定碱可增加成纤维细胞TGF-β1和EGF的自分泌，但对L929成纤维细胞增殖无影响。
结论
紫堇定碱通过增加成纤维细胞TGF-β1和EGF的自分泌促进伤口愈合。
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