Department of Dermatology, Liaocheng People's Hospital, Liaocheng, China.
Department of Plastic & Cosmetic Surgery, The Third Affiliated Hospital of Chongqing Medical University, Chongqing, China.
Bioengineered. 2022 Jan;13(1):789-799. doi: 10.1080/21655979.2021.2012920.
To explore the regulation of keratinocyte growth factor (KGF) in the process of repairing rat skin wounds by taspine hydrochloride (TA/HCl), 45 male Sprague-Dawley (SD) rats were purchased and divided into an experimental group, a dimethyl sulfoxide (DMSO) control group, and a basic fibroblast growth factor (bFGF) control group, each with 15 only. A back trauma model was innovatively adopted to prevent rats from biting and contaminating. The wound healing time and healing rate of the rat, and the Hydroxyproline (Hyp) and KGF expressions were observed. Morphological changes of wound tissue and the number of capillaries were observed after hematoxylin-eosin (HE) staining. The results showed that wound healing rate of experimental group and bFGF group was significantly higher than that of DMSO group ( < 0.05) after 2-15 days, and wound healing time of experimental group was 18 days, which was significantly lower than that of the DMSO group ( < 0.05). Expression levels of Hyp and KGF in the granulation tissue of rats in the experimental group were much higher than those in the DMSO control group after trauma ( < 0.05). In early stage of wound tissue repair, the number of new capillaries formed in experimental group was significantly higher than that in DMSO control group ( < 0.05). In summary, this study innovatively focused on KGF. The mechanism of TA/HCL promoting rat skin wound healing was closely related to KGF.
为了探讨盐酸他司汀（TA/HCl）在修复大鼠皮肤创伤过程中对角质细胞生长因子（KGF）的调控作用，本研究创新性地购买了 45 只雄性 Sprague-Dawley（SD）大鼠，并将其分为实验组、二甲基亚砜（DMSO）对照组和碱性成纤维细胞生长因子（bFGF）对照组，每组 15 只。采用背部创伤模型，以防止大鼠咬伤和污染。观察大鼠的伤口愈合时间和愈合率，羟脯氨酸（Hyp）和 KGF 的表达。苏木精-伊红（HE）染色后观察伤口组织的形态变化和毛细血管数量。结果表明，实验组和 bFGF 组在 2-15 天后的伤口愈合率明显高于 DMSO 组（ < 0.05），实验组的伤口愈合时间为 18 天，明显低于 DMSO 组（ < 0.05）。创伤后实验组大鼠肉芽组织中 Hyp 和 KGF 的表达水平明显高于 DMSO 对照组（ < 0.05）。在伤口组织修复的早期阶段，实验组中新形成的毛细血管数量明显高于 DMSO 对照组（ < 0.05）。综上所述，本研究创新性地聚焦于 KGF，探讨了 TA/HCL 促进大鼠皮肤创伤愈合的机制与 KGF 密切相关。
