[骨髓移植前后患者支气管肺泡灌洗术分析]

[Analysis of bronchoalveolar lavage fluid in patients before and after bone marrow transplantation].

作者信息

Yasui M, Nishi K, Fujimura M, Nomura M, Kurashima K, Shintani H, Sayuri S, Yasuhito N, Matsuda T, Saito M

机构信息

Third Department of Internal Medicine, Kanazawa University School of Medicine, Japan.

出版信息

Nihon Kyobu Shikkan Gakkai Zasshi. 1992 Jan;30(1):51-6.

PMID:1625396

Abstract

We analysed BALF cell findings in 9 patients (group A: 5 patients without lung disease after bone marrow transplantation (BMT), group B: 4 patients with lung disease after BMT) before and after BMT. Before BMT, BALF cell findings in group A were almost normal, whereas a relative increase of lymphocytes was seen in group B. Although total cell counts and cell composition in group A changed little after BMT, CD4/CD8 ratios in BALF lymphocytes decreased. In contrast, a relative increase of lymphocytes and neutrophils was seen in group B and there was variation of CD4/CD8 ratios in BALF lymphocytes after BMT. We there studied the BALF lymphocytes of 3 patients in group A and 4 patients in group B after BMT by means of 2-color analysis. Among CD4+ cell populations, CDw 29+ cells were decreased in both groups after BMT. A relative increase of BALF-CD4+ HLA-DR+ cells and CD8+ HLA-DR+ cells was seen in group A, but was not seen in group B. These findings suggest that there is abnormality of local immunity in the lung after BMT.

摘要

我们分析了9例患者（A组：5例骨髓移植（BMT）后无肺部疾病的患者，B组：4例BMT后有肺部疾病的患者）BMT前后支气管肺泡灌洗（BALF）细胞的检查结果。BMT前，A组的BALF细胞检查结果基本正常，而B组可见淋巴细胞相对增多。虽然A组BMT后细胞总数和细胞组成变化不大，但BALF淋巴细胞中的CD4/CD8比值下降。相比之下，B组可见淋巴细胞和中性粒细胞相对增多，且BMT后BALF淋巴细胞中的CD4/CD8比值存在变化。我们随后通过双色分析研究了BMT后A组3例患者和B组4例患者的BALF淋巴细胞。在CD4+细胞群体中，两组BMT后CDw29+细胞均减少。A组可见BALF-CD4+HLA-DR+细胞和CD8+HLA-DR+细胞相对增多，而B组未见此现象。这些发现提示BMT后肺部存在局部免疫异常。