Loring Jeanne F, Rao Mahendra S
Stem Cell Center, Burnham Institute for Medical Research, La Jolla, CA 92037, USA.
Stem Cells. 2006 Jan;24(1):145-50. doi: 10.1634/stemcells.2005-0432. Epub 2005 Oct 27.
As of August 2005, 22 human embryonic stem cell (hESC) lines listed on the National Institutes of Health (NIH) hESC Registry were being distributed to investigators. At a June 2005 meeting of NIH-supported hESC researchers, we proposed that a set of shared standards should be available in order to characterize the cells unambiguously in multiple laboratories. Here, we elaborate such a plan to identify a set of standard methods and to initiate collaborative efforts to validate the standards. The standard assays we propose should be comprehensive enough to ensure that hESC banks can provide a consistent and reliable product for NIH researchers, and inexpensive enough that individual laboratories can afford to use at least some of the methods routinely in their laboratories. We expect that as data accumulate and standards evolve, a core set of tests will become the norm for routine assessment of hESC cultures and that these tests will lay the groundwork for clinical applications of these cells.
截至2005年8月，美国国立卫生研究院（NIH）人类胚胎干细胞（hESC）登记处列出的22个人类胚胎干细胞系正被分发给研究人员。在2005年6月由NIH资助的hESC研究人员会议上，我们提议应制定一套共享标准，以便在多个实验室中明确鉴定这些细胞。在此，我们详细阐述这样一个计划，以确定一套标准方法并启动合作努力来验证这些标准。我们提议的标准检测方法应足够全面，以确保hESC库能够为NIH研究人员提供一致且可靠的产品，同时成本要足够低，以便各个实验室能够负担得起在其实验室中常规使用至少一些方法。我们预计，随着数据的积累和标准的不断完善，一组核心检测将成为hESC培养常规评估的规范，并且这些检测将为这些细胞的临床应用奠定基础。
