Jun Mira, Hong Jungil, Jeong Woo-Sik, Ho Chi-Tang
Department of Food Science, Rutgers University, 65 Dudley Road, New Brunswick, NJ 08901-8520, USA.
Mol Nutr Food Res. 2005 Dec;49(12):1154-9. doi: 10.1002/mnfr.200500103.
Inhibitory effect of kudzu isoflavones on arachidonic acid metabolism and nitric oxide (NO) production in lipopolysaccharide activated RAW 264.7 macrophages were investigated. Isoflavone aglycones, such as daidzein, genistein, biochanin A, and formononetin significantly suppressed arachidonic acid release (50 microM). Biochanin A, which displayed the most active inhibition on arachidonic acid release in HT-29 human colon cancer cells, exhibited its most potent suppression in RAW 264.7 cell (by 86%) without showing cytotoxicity. However, isoflavone glucosides, puerarin and daidzin, showed lower inhibitory activities on the release of arachidonic acid and its metabolites. In NO formation, biochanin A showed marked inhibition, by 62% (50 microM), followed by genistein, daidzein, formononetin, and daidzin, 56, 39, 33, and 8%, respectively. 5,7-Dihydroxyl group in the A-ring of isoflavones could be a key functional group responsible for the strong inhibitory activity of biochanin A and genistein on NO production. These activities may contribute to the antiinflammatory and anticarcinogenic properties of kudzu isoflavones.
研究了葛根异黄酮对脂多糖激活的RAW 264.7巨噬细胞中花生四烯酸代谢和一氧化氮（NO）生成的抑制作用。大豆苷元、染料木黄酮、鹰嘴豆芽素A和芒柄花黄素等异黄酮苷元显著抑制花生四烯酸释放（50微摩尔）。鹰嘴豆芽素A在HT-29人结肠癌细胞中对花生四烯酸释放表现出最活跃的抑制作用，在RAW 264.7细胞中表现出最强的抑制作用（86%），且无细胞毒性。然而，异黄酮糖苷、葛根素和大豆苷对花生四烯酸及其代谢产物的释放抑制活性较低。在NO生成方面，鹰嘴豆芽素A表现出显著抑制作用，抑制率为62%（50微摩尔），其次是染料木黄酮、大豆苷元、芒柄花黄素和大豆苷，分别为56%、39%、33%和8%。异黄酮A环上的5,7-二羟基可能是鹰嘴豆芽素A和染料木黄酮对NO生成具有强抑制活性的关键官能团。这些活性可能有助于葛根异黄酮的抗炎和抗癌特性。
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