Angeles Sánchez-Sampedro María, Fernández-Tárrago Jorge, Corchete Purificación
Departamento de Fisiología Vegetal, Facultad de Farmacia, Universidad de Salamanca, Campus Miguel de Unamuno, Spain.
J Plant Physiol. 2005 Oct;162(10):1177-82. doi: 10.1016/j.jplph.2005.01.012.
Elimination of calcium ions from the medium of cell cultures of Silybum marianum (L.) Gaertn increased flavonolignan production. Silymarin accumulation was not altered by treatment of cultures with the calcium ionophore A23187. The specific Ca2+ chelator, EGTA, enhanced the silymarin content in cells by 200%, and its secretion by 3-4 times. The inorganic ion La3+, as well as the calcium channel inhibitor verapamil, also stimulated production. Several reagents known to block intracellular calcium movement, such as ruthenium red, thapsigargin and TMB-8 appreciably increased silymarin accumulation. These results suggest that inhibition of external and internal calcium fluxes plays a significant role in flavonolignan metabolism of S. marianum cell cultures.
从水飞蓟（Silybum marianum (L.) Gaertn）细胞培养介质中去除钙离子可增加黄酮木脂素的产量。用钙离子载体A23187处理培养物，水飞蓟素的积累没有改变。特异性Ca2+螯合剂乙二醇双四乙酸（EGTA）使细胞中的水飞蓟素含量提高了200%，其分泌量提高了3至4倍。无机离子La3+以及钙通道抑制剂维拉帕米也刺激了产量。几种已知可阻断细胞内钙移动的试剂，如钌红、毒胡萝卜素和TMB-8，显著增加了水飞蓟素的积累。这些结果表明，抑制细胞内外钙通量在水飞蓟细胞培养物的黄酮木脂素代谢中起重要作用。
