持续去极化可诱导大鼠胚胎海马与隔区共培养神经元中的胆碱乙酰转移酶活性。

Continuous depolarization induces choline acetyltransferase activity in septal and hippocampal co-cultured embryonic rat neurons.

作者信息

Suzuki Takeshi, Shimotohno Kumiko

机构信息

Department of Basic Biological Sciences, Kyoritsu University of Pharmacy, 1-5-30 Shiba-Koen, Minato-ku, Tokyo 105-8512, Japan.

出版信息

Neurosci Lett. 2006 Feb 6;394(1):79-81. doi: 10.1016/j.neulet.2005.10.017. Epub 2005 Oct 26.

DOI:10.1016/j.neulet.2005.10.017

Abstract

We tested the influence of continuous high-K+ treatment on acetylcholine (ACh) release and choline acetyltransferase (ChAT) activity on septal cell culture, and septal and hippocampal cell co-culture obtained from rat embryos. Continuous 9 mM K+ treatment did not affect ACh release and ChAT activity in septal culture, but increased ACh release in co-culture without affecting ChAT activity. A slight increase in extracellular K+ concentration, therefore, induced neuronal excitation. Continuous 55 mM K+ treatment increased ACh release in septal culture. This effect was due to direct excitation of septal neurons. In co-culture, 55 mM K+ treatment increased both ACh release and ChAT activity. These results indicate that hippocampal neurons are indispensable for the depolarization-induced increase in ChAT activity in the early stage of developing septal cholinergic neurons.

摘要

我们测试了持续高钾处理对大鼠胚胎来源的隔区细胞培养物以及隔区与海马细胞共培养物中乙酰胆碱（ACh）释放和胆碱乙酰转移酶（ChAT）活性的影响。持续9 mM钾处理不影响隔区培养物中的ACh释放和ChAT活性，但在共培养物中增加了ACh释放，而不影响ChAT活性。因此，细胞外钾浓度的轻微升高会诱导神经元兴奋。持续55 mM钾处理增加了隔区培养物中的ACh释放。这种效应是由于隔区神经元的直接兴奋。在共培养物中，55 mM钾处理增加了ACh释放和ChAT活性。这些结果表明，在发育中的隔区胆碱能神经元早期，海马神经元对于去极化诱导的ChAT活性增加是不可或缺的。