Björklund A, Gage F H, Schmidt R H, Stenevi U, Dunnett S B
Acta Physiol Scand Suppl. 1983;522:59-66.
The time-course and magnitude of fibre outgrowth from septal suspension grafts injected into the previously denervated hippocampal formation was monitored by measurements of choline acetyltransferase (ChAT), and the activity of the grafted neurons was assessed by measurements of [14C]acetylcholine (ACh) synthesis from [14C]glucose in vitro. Graft-derived ChAT activity was barely detectable 10 days after grafting, but increased sharply between 10 days and 1 month in the areas of the hippocampus located close to the septal implants. By 6 months ChAT activity was restored to near normal levels in all segments of the previously denervated hippocampus. The overall hippocampal [14C]ACh synthesis was also restored to normal levels in the grafted animals, and estimates of the ACh turnover rate suggested that the transmitter machinery of the newly established "septo-hippocampal" connections operated at a rate similar to that of the intrinsic septohippocampal pathway. The intrahippocampal septal suspension grafts, similar to the intrastriatal nigral grafts, thus seem to be capable of maintaining function at a relatively "physiological" level despite their abnormal positions.
通过测量胆碱乙酰转移酶（ChAT）来监测注入先前去神经支配的海马结构的中隔悬浮移植物中纤维生长的时间进程和程度，并通过体外测量[14C]葡萄糖合成[14C]乙酰胆碱（ACh）来评估移植神经元的活性。移植后10天，移植来源的ChAT活性几乎检测不到，但在靠近中隔植入物的海马区域，10天至1个月之间急剧增加。到6个月时，先前去神经支配的海马所有节段的ChAT活性恢复到接近正常水平。移植动物海马整体的[14C]ACh合成也恢复到正常水平，ACh周转率的估计表明，新建立的“中隔-海马”连接的递质机制运行速率与内在的中隔-海马通路相似。因此，海马内中隔悬浮移植物与纹状体内黑质移植物相似，尽管位置异常，但似乎能够在相对“生理”的水平维持功能。
