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基于线粒体细胞色素b基因序列变异的家鹅品种系统发育关系

[Phylogenetic relationships among domestic goose breeds based on mitochondrial cytochrome b gene sequence variation].

作者信息

Wang Ji-Wen, Liu An-Fang, Chen Yan-Rong, Qu Hao

机构信息

College of Animal Science and Technology, Sichuan Agricultural University, Ya'an 625014, China.

出版信息

Yi Chuan. 2005 Sep;27(5):741-6.

Abstract

The mitochondrial cytochrome b (cyt b) gene of 17 breeds of domestic goose (2 in Europe and 15 in China) was sequenced and compared with that of the representative of wild species Anser albifrons. Sequence analysis revealed that thirty-one variable sites and 4 different haplotypes were found among the 45 sequences examined, and nucleotide diversity and haplotype diversity were 0.0068 and 0.45, respectively. Mutations of insertion/deletion were not found. Across all sites,the abundance of G was low (14.2%), whereas the percentages of A, T, and C were quite similar among three Anser goose species. The frequency of guanine differs greatly among the three codon positions. Compositional bias is smaller at first and second positions (0.057 and 0.223) than at third positions (0.492), the probability of transition was higher than that of tranversion (Ts/Tv=9.5-19), there was the highest number of transition in the third codon. The phylogenetic analysis support the hypothesis of dual origin of domestic geese.

摘要

对17个家鹅品种(欧洲2个品种,中国15个品种)的线粒体细胞色素b(cyt b)基因进行了测序,并与野生物种白额雁的代表品种进行了比较。序列分析显示,在所检测的45个序列中发现了31个可变位点和4种不同的单倍型,核苷酸多样性和单倍型多样性分别为0.0068和0.45。未发现插入/缺失突变。在所有位点中,鸟嘌呤(G)的丰度较低(14.2%),而在三种雁属鹅种中,腺嘌呤(A)、胸腺嘧啶(T)和胞嘧啶(C)的百分比相当相似。鸟嘌呤在三个密码子位置的频率差异很大。第一和第二位点的组成偏差(0.057和0.223)小于第三位点(0.492),转换概率高于颠换概率(Ts/Tv = 9.5 - 19),第三位密码子的转换数量最多。系统发育分析支持家鹅双重起源的假说。

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