Gemba T, Matsunaga K, Ueda M
Shionogi Research Laboratories, Shionogi & Co., Ltd., Osaka, Japan.
Neurosci Lett. 1992 Feb 3;135(2):184-8. doi: 10.1016/0304-3940(92)90432-7.
To investigate the role of glutamate release in cerebral ischemia, the amounts of amino acids (glutamate, taurine, alanine, glycine and glutamine) released in the hippocampal CA1 region of stroke-prone SHR (SHRSP), stroke-resistant SHR (SHRSR) and normotensive rats (WKY) were determined during and after cerebral ischemia by the microdialysis method under halothane anesthesia. Cerebral ischemia was produced by the occlusion of both common carotid arteries for 20 min. The basal amino acids release did not differ among the three strains of rats, but ischemic glutamate and taurine releases were more marked in SHRSP than in other strains. These results suggest that the massive glutamate release during cerebral ischemia of SHRSP might be related with severe neuronal cell injury.
为研究谷氨酸释放在脑缺血中的作用，采用微透析法在氟烷麻醉下，测定了易卒中自发性高血压大鼠（SHRSP）、抗卒中自发性高血压大鼠（SHRSR）和正常血压大鼠（WKY）海马CA1区在脑缺血期间及缺血后的氨基酸（谷氨酸、牛磺酸、丙氨酸、甘氨酸和谷氨酰胺）释放量。通过阻断双侧颈总动脉20分钟造成脑缺血。三种品系大鼠的基础氨基酸释放量无差异，但SHRSP缺血时谷氨酸和牛磺酸的释放比其他品系更明显。这些结果表明，SHRSP脑缺血期间大量谷氨酸释放可能与严重的神经元细胞损伤有关。
