Mazzei M, Carrozza M L, Bandecchi P, Mazzanti G, Mannelli A, Tolari F
Dipartimento di Patologia Animale, Profilassi ed Igiene degli Alimente, Università di Pisa, Via delle Piagge 2, 56124 Pisa, Italy.
Vet Rec. 2005 Oct 29;157(18):552-5. doi: 10.1136/vr.157.18.552.
An elisa was used to detect antibodies to maedi-visna virus in samples of serum and milk from individual sheep; the results obtained indicated that the elisa can be used to detect antibodies in milk. The assay was also applied to samples of bulk-tank milk; a standard curve was created and used to calculate the seroprevalence of maedi-visna in 11 flocks of sheep and the results were compared with the results obtained by applying the elisa to individual serum samples. There was good agreement between the seroprevalences calculated from the standard curve for bulk-tank milk and from the individual serum samples.
采用酶联免疫吸附测定法（ELISA）检测个体绵羊血清和乳汁样本中的梅迪-维斯纳病毒抗体；所得结果表明，ELISA可用于检测乳汁中的抗体。该检测方法还应用于储存槽牛奶样本；绘制了标准曲线，并用于计算11群绵羊中梅迪-维斯纳病的血清阳性率，且将结果与对个体血清样本应用ELISA所得结果进行比较。根据储存槽牛奶标准曲线计算出的血清阳性率与个体血清样本计算出的血清阳性率之间具有良好的一致性。
