Zanoni R G, Vogt H R, Pohl B, Böttcher J, Bommeli W, Peterhans E
Institute of Veterinary Virology, Bern, Switzerland.
Zentralbl Veterinarmed B. 1994 Dec;41(10):662-9. doi: 10.1111/j.1439-0450.1994.tb00277.x.
A new ELISA kit was developed, based on highly purified whole-virus antigen derived from the Swiss maedi-visna virus strain OLV. The sensitivity, specificity and accuracy of this assay were compared with that of an established ELISA based on recombinant GAG (group-specific antigens)-GST (glutathione S-transferase) fusion protein expressed in E. coli (GAG-GST ELISA). The whole-virus ELISA exhibits at least comparable specificity (99.3%) but higher sensitivity (98.6 versus 86.3%) and agreement with the 'true' status beyond chance in the detection of antiviral antibodies in serum from goats. Antibodies in milk samples are detected with higher specificity (98.9 versus 97.8%) but lower sensitivity (91.4 versus 98.2%) than in GAG-GST ELISA. The specificity of the new ELISA in the detection of antibodies in serum might be superior, since a set of 40 samples falsely rated positive in GAG-GST ELISA in routine diagnostic work was negative in the new ELISA. In both assays, milk samples can be tested instead of serum, although with slightly reduced sensitivity in the new ELISA. The major advantage of the new test kit is the low number of equivocal samples needing confirmation in a supplementary test. Results obtained with sheep sera indicate that the new ELISA kit is also suitable for the detection of antibodies to maedi-visna virus.
基于源自瑞士梅迪-维斯纳病毒株OLV的高度纯化全病毒抗原，开发了一种新的酶联免疫吸附测定（ELISA）试剂盒。将该检测方法的灵敏度、特异性和准确性与基于在大肠杆菌中表达的重组GAG（群特异性抗原）-GST（谷胱甘肽S-转移酶）融合蛋白的既定ELISA方法（GAG-GST ELISA）进行了比较。全病毒ELISA显示出至少相当的特异性（99.3%），但灵敏度更高（98.6%对86.3%），并且在检测山羊血清中的抗病毒抗体时与“真实”状态的一致性超过偶然概率。与GAG-GST ELISA相比，牛奶样本中的抗体检测特异性更高（98.9%对97.8%），但灵敏度更低（91.4%对98.2%）。新ELISA在血清抗体检测中的特异性可能更高，因为在常规诊断工作中，一组在GAG-GST ELISA中被错误判定为阳性的40个样本在新ELISA中呈阴性。在这两种检测方法中，都可以检测牛奶样本而非血清，不过新ELISA的灵敏度略有降低。新检测试剂盒的主要优点是需要在补充检测中进行确认的疑似样本数量较少。用绵羊血清获得的结果表明，新ELISA试剂盒也适用于检测梅迪-维斯纳病毒抗体。
