核糖苷链霉菌中核糖霉素生物合成基因簇：与布替罗星生物合成的比较。
The ribostamycin biosynthetic gene cluster in Streptomyces ribosidificus: comparison with butirosin biosynthesis.
作者信息
Subba Bimala, Kharel Madan Kumar, Lee Hei Chan, Liou Kwangkyoung, Kim Byung-Gee, Sohng Jae Kyung
机构信息
Institute of Biomolecule Reconstruction, Sun Moon University, Asan 336-708, Korea.
出版信息
Mol Cells. 2005 Aug 31;20(1):90-6.
A cluster of genes for ribostamycin (Rbm) biosynthesis was isolated from Streptomyces ribosidificus ATCC 21294. Sequencing of 31.892 kb of the genomic DNA of S. ribosidificus revealed 26 open reading frames (ORFs) encoding putative Rbm biosynthetic genes as well as resistance and other genes. One of ten putative Rbm biosynthetic genes, rbmA, was expressed in S. lividans TK24, and shown to encode 2-deoxy-scyllo-inosose (DOI) synthase. Acetylation of various aminoglycoside-aminocyclitol (AmAcs) by RbmI confirmed it to be an aminoglycoside 3-N-acetyltransferase. Comparison of the genetic control of ribostamycin and butirosin biosynthesis pointed to a common biosynthetic route for these compounds, despite the considerable differences between them in genetic organization.
从产核糖霉素链霉菌ATCC 21294中分离出了一组核糖霉素（Rbm）生物合成基因。对产核糖霉素链霉菌31.892 kb基因组DNA的测序揭示了26个开放阅读框（ORF），这些开放阅读框编码推定的Rbm生物合成基因以及抗性基因和其他基因。十个推定的Rbm生物合成基因之一rbmA在变铅青链霉菌TK24中表达，并显示编码2-脱氧-scyllo-肌醇（DOI）合酶。RbmI对各种氨基糖苷-氨基环醇（AmAcs）的乙酰化作用证实它是一种氨基糖苷3-N-乙酰基转移酶。尽管核糖霉素和丁胺卡那霉素在基因组织上存在相当大的差异，但对它们生物合成的遗传控制比较表明这些化合物有共同的生物合成途径。
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