在不产生氨基糖苷类抗生素的变铅青链霉菌TK24中生产氨基糖苷类抗生素。
Production of aminoglycosides in non-aminoglycoside producing Streptomyces lividans TK24.
作者信息
Subba Bimala, Kurumbang Nagendra Prasad, Jung Young Soo, Yoon Yeo Joon, Lee Hei Chan, Liou Kwangkyoung, Sohng Jae Kyung
机构信息
Institute of Biomolecule Reconstruction (IBR), Department of Pharmaceutical Engineering, Sun Moon University, #100, Kalsan-ri, Tangjeong-myeon, Asansi, Chungnam 336-708, Republic of Korea.
出版信息
Bioorg Med Chem Lett. 2007 Apr 1;17(7):1892-6. doi: 10.1016/j.bmcl.2007.01.035. Epub 2007 Jan 19.
The pRBM4 cosmid, which harbors a putative cluster of genes spanning a 31.8-kb chromosomal region of the ribostamycin producer Streptomyces ribosidificus ATCC 21294, was heterologously expressed in Streptomyces lividans TK24. ESI-MS/MS, HPLC, and LC-ESI MS analyses showed that the transformation gave rise to ribostamycin production in various culture broths. This is the first report of heterologous aminoglycoside production.
携带推测的基因簇的pRBM4黏粒，该基因簇跨越核糖霉素产生菌嗜盐链霉菌ATCC 21294的一个31.8 kb染色体区域，在变铅青链霉菌TK24中进行了异源表达。电喷雾串联质谱（ESI-MS/MS）、高效液相色谱（HPLC）和液相色谱-电喷雾质谱（LC-ESI MS）分析表明，该转化在各种培养液中产生了核糖霉素。这是关于异源氨基糖苷生产的首次报道。
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