Alternative patterns of MHC-restricted antibody responsiveness following intraperitoneal immunization of inbred mice with different preparations of an 86 kilodalton antigen of Schistosoma mansoni.

The investigation of MHC restricted antibody responses to an 86 kDa antigen (p86) during chronic Schistosoma mansoni infection has been extended to immunization with this antigen. In the absence of adjuvant, a similar pattern of responsiveness by mice expressing H-2k and H-2d but not H-2b was observed following immunization with unpurified adult worm homogenate. Adjuvant selectively abrogated the capacity of H-2d mice to respond and this was also the case when purified p86 with adjuvant was injected. Immunization with purified subfragments of p86 again demonstrated MHC restriction in the capacity to immunoprecipitate p86 in vitro translation product, the pattern varying according to the fragment used. Western blot analysis showed that in some, but not all instances of apparent 'non-responsiveness' characterized by immunoprecipitation, antibody specificities capable of recognizing p86 epitopes on the nitrocellulose bound p86 were, indeed, present. Thus the fine specificity as well as the absolute capacity to respond is influenced by both the MHC haplotype of the host and the nature of the immunizing antigen.