抗坏血酸对化学分析物的干扰。

Interference of ascorbic acid with chemical analytes.

作者信息

Meng Qing H, Irwin William C, Fesser Jennifer, Massey K Lorne

机构信息

Department of Pathology, Division of Clinical Biochemistry, Royal University Hospital, College of Medicine, University of Saskatchewan, Saskatoon, SK, S7N 0W8, Canada.

出版信息

Ann Clin Biochem. 2005 Nov;42(Pt 6):475-7. doi: 10.1258/000456305774538274.

DOI:10.1258/000456305774538274

PMID:16259800

Abstract

BACKGROUND

Ascorbic acid can interfere with methodologies involving redox reactions, while comprehensive studies on main chemistry analysers have not been reported. We therefore attempted to determine the interference of ascorbic acid with analytes on the Beckman Synchron LX20.

METHODS

Various concentrations of ascorbic acid were added to serum, and the serum analytes were measured on the LX20.

RESULTS

With a serum ascorbic acid concentration of 12.0 mmol/L, the values for sodium, potassium, calcium and creatinine increased by 43%, 58%, 103% and 26%, respectively (P<0.01). With a serum ascorbic acid concentration of 12.0 mmol/L, the values for chloride, total bilirubin and uric acid decreased by 33%, 62% and 83%, respectively (P<0.01), and were undetectable for total cholesterol, triglyceride, ammonia and lactate. There was no definite influence of ascorbic acid on analytical values for total CO(2), urea, glucose, phosphate, total protein, albumin, amylase, creatine kinase, creatine kinase-MB, aspartate aminotransferase, alanine aminotransferase, alkaline phosphatase, total iron, unbound iron-binding capacity or magnesium.

CONCLUSIONS

Ascorbic acid causes a false increase in sodium, potassium, calcium and creatinine results and a false decrease in chloride, total bilirubin, uric acid, total cholesterol, triglyceride, ammonia and lactate results.

摘要

背景

抗坏血酸可干扰涉及氧化还原反应的方法，然而尚未见关于主要化学分析仪的全面研究报道。因此，我们试图确定抗坏血酸对贝克曼库尔特同步LX20分析仪上分析物的干扰情况。

方法

向血清中加入不同浓度的抗坏血酸，然后在LX20分析仪上测定血清分析物。

结果

血清抗坏血酸浓度为12.0 mmol/L时，钠、钾、钙和肌酐的值分别升高了43%、58%、103%和26%（P<0.01）。血清抗坏血酸浓度为12.0 mmol/L时，氯、总胆红素和尿酸的值分别降低了33%、62%和83%（P<0.01），总胆固醇、甘油三酯、氨和乳酸无法检测到。抗坏血酸对总二氧化碳、尿素、葡萄糖、磷酸盐、总蛋白、白蛋白、淀粉酶、肌酸激酶、肌酸激酶同工酶MB、天冬氨酸氨基转移酶、丙氨酸氨基转移酶、碱性磷酸酶、总铁、未结合铁结合力或镁的分析值没有明确影响。