Fitó M, Biosca C, Hernandez J M, Galimany R
Department of Clinical Biochemistry, Hospital Germans Trias i Pujol, Ctra. de Canyet s/n, Badalona, Barcelona, Spain.
Eur J Clin Chem Clin Biochem. 1997 Oct;35(10):787-92.
The increasing availability and use of automatic analysers in clinical chemistry have revealed a number of endogenous interferences. We evaluated the effect of bilirubin, haemolysis and lipaemia on the Falcor-600 analytical system (Menarini Diagnostics) and the Dax-48 (Bayer Diagnostic). We studied the potential endogenous interferences in the measurement of serum glucose, urea, creatinine, cholesterol, triacylglycerols, total bilirubin, total protein, aspartate aminotransferase, alanine aminotransferase and gamma-glutamyltransferase on both analysers; and albumin, direct bilirubin, uric acid, inorganic phosphorus, iron, calcium, magnesium, chloride, sodium, potassium, alkaline phosphatase, amylase, lactate dehydrogenase and creatine kinase on the Dax-48. We followed the guidelines of the Spanish Society of Clinical Biochemistry and Molecular Pathology. Bilirubin samples were prepared using bovine bilirubin, and studied in the concentration range of 20 to 400 mumol/l. For haemolysis, the pool was spiked with a diluted haemolysate of human red cells to achieve a concentration range of 10 to 120 mumol/l of haemoglobin. Lipaemia was studied using samples spiked with Intralipid, a fat emulsion, at concentrations from 1 g/l to 6 g/l (3 to 18 mumol/l of triacylglycerols).
临床化学中自动分析仪的可用性和使用日益增加，已揭示出一些内源性干扰。我们评估了胆红素、溶血和脂血对Falcor - 600分析系统（美纳里尼诊断公司）和Dax - 48（拜耳诊断公司）的影响。我们研究了这两种分析仪在测定血清葡萄糖、尿素、肌酐、胆固醇、三酰甘油、总胆红素、总蛋白、天冬氨酸氨基转移酶、丙氨酸氨基转移酶和γ-谷氨酰转移酶时潜在的内源性干扰；以及Dax - 48在测定白蛋白、直接胆红素、尿酸、无机磷、铁、钙、镁、氯、钠、钾、碱性磷酸酶、淀粉酶、乳酸脱氢酶和肌酸激酶时的潜在内源性干扰。我们遵循了西班牙临床生物化学和分子病理学学会的指南。胆红素样本使用牛胆红素制备，研究浓度范围为20至400μmol/l。对于溶血，向混合样本中加入稀释的人红细胞溶血产物，使血红蛋白浓度范围达到10至120μmol/l。使用加入脂肪乳剂英脱利匹特的样本研究脂血，浓度范围为1 g/l至6 g/l（三酰甘油为3至18μmol/l）。
