Santucci Daniela, Sorace Alberto, Francia Nadia, Aloe Luigi, Alleva Enrico
Section of Behavioural Neurosciences, Department of Cell Biology and Neurosciences, Istituto Superiore di Sanità, I-00161 Rome, Italy.
Behav Brain Res. 2006 Jan 6;166(1):124-30. doi: 10.1016/j.bbr.2005.07.032. Epub 2005 Nov 2.
The long-term effects on isolation-induced aggressive behaviour and central NGF and BDNF levels of gestational exposures to ozone (O(3)) were evaluated in adult CD-1 mice. Females were exposed to O(3), at the dose of 0.0, 0.3 or 0.6 ppm from 30 days prior the formation of breeding pairs until gestational day 17. Litters were fostered at birth to untreated dams and, at adulthood, male offspring underwent five successive daily encounters (15 min each) with a standard opponent of the same strain, sex, weight and age. The encounters on day 1, 3 and 5 were videotaped and agonistic and non-agonistic behavioural items finely scored. O(3)-exposed mice showed a significant increase in freezing and defensive postures, a decrease in nose-sniffing behaviour and reduced progressively the aggressive behavioural profile displayed on day 1. Reduced NGF levels in the hippocampus and increased BDNF in the striatum were also found upon O(3) exposure.
在成年CD-1小鼠中评估了孕期暴露于臭氧（O₃）对隔离诱导的攻击行为以及中枢神经生长因子（NGF）和脑源性神经营养因子（BDNF）水平的长期影响。从配对前30天到妊娠第17天，雌性小鼠暴露于浓度为0.0、0.3或0.6 ppm的O₃环境中。幼崽出生后由未处理的母鼠抚养，成年后，雄性后代每天与同品系、同性、体重和年龄相同的标准对手连续进行五次接触（每次15分钟）。第1、3和5天的接触过程进行录像，并对攻击性行为和非攻击性行为项目进行详细评分。暴露于O₃的小鼠表现出僵住和防御姿势显著增加，嗅鼻行为减少，且在第1天表现出的攻击性行为模式逐渐减少。暴露于O₃后还发现海马体中NGF水平降低，纹状体中BDNF水平升高。
