Matz Mikhail V, Meleshkevitch Ella A
Whitney Laboratory for Marine Biology, University of Florida, St. Augustine, USA.
Methods Mol Biol. 2006;317:59-74. doi: 10.1385/1-59259-968-0:059.
Ordered differential display (ODD) is one of the approaches that uses systematic, rather than random, sampling of transcripts for display and thereby provides means to browse through essentially all the transcripts in the compared mRNA pools. It is specifically adapted for small amounts of starting material. The protocol outlined here, in addition to ODD procedure itself, also describes isolation of RNA and synthesis of double-stranded cDNA from small biological samples.
有序差异显示(ODD)是一种利用对转录本进行系统而非随机抽样来进行显示的方法,从而提供了浏览比较的mRNA库中几乎所有转录本的手段。它特别适用于少量起始材料。这里概述的方案,除了ODD程序本身外,还描述了从小生物样本中分离RNA和合成双链cDNA的方法。