通过差异显示检测mRNA多态性。

Detection of an mRNA polymorphism by differential display.

作者信息

Liang Shan, Rossby S Paul, Liang Peng, Shelton Richard C, Manier D Hal, Chakrabarti Amitabha, Sulser Fridolin

机构信息

Department of Psychiatry, Vanderbilt University Medical Center, Nashville, TN, USA.

出版信息

Methods Mol Biol. 2006;317:279-85. doi: 10.1385/1-59259-968-0:279.

DOI:10.1385/1-59259-968-0:279

PMID:16264236

Abstract

Differential display technology was utilized to compare programs of gene expression in primary cultures of human skin fibroblasts from normal volunteers and patients diagnosed with melancholic depression. Polymorphic transcripts of a single gene differing by one tandem repeat sequence of four nucleotides (TGAT) in the 3' noncoding region were detected.

摘要

利用差异显示技术比较正常志愿者和被诊断为忧郁症的患者的人皮肤成纤维细胞原代培养物中的基因表达程序。检测到一个单基因的多态性转录本，其在3'非编码区有一个由四个核苷酸（TGAT）组成的串联重复序列差异。