Zamora M, Samal S K
Regional College of Veterinary Medicine, University of Maryland, College Park 20742.
Virus Res. 1992 Jun;24(1):115-21. doi: 10.1016/0168-1702(92)90035-8.
The nucleotide sequences of seven gene junctions (N-P, P-M, M-SH, SH-G, G-F, F-M2 and M2-L) of bovine respiratory syncytial virus (BRSV) strain A51908 were determined by dideoxynucleotide sequencing of cDNAs from polytranscript mRNAs and from genomic RNA. By comparison with the consensus sequences derived from human respiratory syncytial virus (HRSV) mRNAs, gene-start and gene-end sequences were found in all BRSV mRNAs. There was a perfect match between the BRSV and HRSV in all gene-start sequences, except for the sequence of the SH gene which contained one nucleotide difference compared to HRSV A2; and the gene-start sequence of the L gene, which was one nucleotide shorter than the corresponding sequence of HRSV. Analysis of the intergenic regions showed a high degree of divergence in the nucleotide sequence between BRSV and HRSV. However, the length of the nucleotides in the intergenic sequences was similar for a given gene junction. As in the case of HRSV, the M2 and L genes of BRSV overlap by 68 nucleotides, suggesting a similar transcription attenuation mechanism. The sequences of the overlap, corresponding to the 3' end of the L gene, were almost identical between BRSV and HRSV.
通过对多转录本mRNA和基因组RNA的cDNA进行双脱氧核苷酸测序，确定了牛呼吸道合胞病毒（BRSV）A51908株七个基因连接处（N-P、P-M、M-SH、SH-G、G-F、F-M2和M2-L）的核苷酸序列。通过与源自人类呼吸道合胞病毒（HRSV）mRNA的共有序列进行比较，在所有BRSV mRNA中发现了基因起始和基因终止序列。除了SH基因的序列与HRSV A2相比有一个核苷酸差异，以及L基因的基因起始序列比HRSV的相应序列短一个核苷酸外，BRSV和HRSV的所有基因起始序列完全匹配。基因间区域分析显示，BRSV和HRSV之间的核苷酸序列存在高度差异。然而，对于给定的基因连接处，基因间序列中核苷酸的长度相似。与HRSV一样，BRSV的M2和L基因重叠68个核苷酸，表明转录衰减机制相似。BRSV和HRSV之间对应于L基因3'端的重叠序列几乎相同。
